MAGI1 Polyclonal antibody

• 

  WB analysis using 55048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55048-1-AP (MAGI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55048-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55048-1-AP (MAGI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 55048-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55048-1-AP (MAGI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

MAGI1 Polyclonal Antibody for WB, ELISA

Cat No. 55048-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AIP 3, AIP3, BAI1 associated protein 1, BAIAP1, BAP 1, BAP1, MAGI 1, MAGI1, TNRC19, WW domain containing protein 3, WWP3

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, mouse kidney tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

55048-1-AP targets MAGI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 160-170 kDa, 110-120 kDa
• GenBank Accession Number: NM_004742
• Gene Symbol: MAGI1
• Gene ID (NCBI): 9223
• RRID: AB_10858485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96QZ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGI1, also named as AIP3, BAIAP1, BAP1 and TNRC19, may play a role as scaffolding protein at cell-cell junctions. MAGI1 regulates acid-induced ACCN3 currents by modulating its expression at the cell surface. The antibody is specific to MAGI1. The antibody can recognize all the isoform of MAGI1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Cell Dev Biol; Long Non-coding RNA TMEM220-AS1 Suppressed Hepatocellular Carcinoma by Regulating the miR-484/MAGI1 Axis as a Competing Endogenous RNA.; Authors - Cong Cao ; KD Validated
mouse	WB	J Cell Mol Med; Phosphorylation-mediated PI3K-Art signalling pathway as a therapeutic mechanism in the hydrogen-induced alleviation of brain injury in septic mice; Authors - Yuanyuan Bai