Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11660-1-AP targets MALT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2182 Product name: Recombinant human MALT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-450 aa of BC030143 Sequence: GIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRSVDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYMVPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIDELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLL Predict reactive species |
| Full Name | mucosa associated lymphoid tissue lymphoma translocation gene 1 |
| Calculated Molecular Weight | 824 aa, 92 kDa |
| Observed Molecular Weight | 92-100 kDa |
| GenBank Accession Number | BC030143 |
| Gene Symbol | MALT1 |
| Gene ID (NCBI) | 10892 |
| RRID | AB_2139135 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UDY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MALT1 antibody 11660-1-AP | Download protocol |
| IHC protocol for MALT1 antibody 11660-1-AP | Download protocol |
| WB protocol for MALT1 antibody 11660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MALT1 antibody (Cat# 11660-1-AP) has 17 total citations across 15 journals including Cell Reports, Antioxidants & Redox Signaling, European Journal of Pharmacology, ACS Chemical Neuroscience, and Molecular Neurobiology. Research fields span cardiac ischemia/reperfusion injury, pyroptosis, ferroptosis, necroptosis, oxidative stress, stroke neuroprotection, Alzheimer's disease, cancer (breast and prostate), intestinal inflammation, pulmonary fibrosis, and infectious disease.
| Species | Application | Title |
|---|---|---|
Gut Microbes Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage. | ||
Acta Pharmacol Sin Long non-coding RNA STMN1P2 promotes breast cancer doxorubicin resistance by inhibiting pyroptosis through the hnRNPU-EZH2-TARF6-MALT1-caspase-1 pathway | ||
Cell Rep Lactobacillus delbrueckii surface protein P4430 attenuates intestinal inflammation by modulating macrophage polarization via Mincle. | ||
Antioxid Redox Signal CARD11-BCL10-MALT1 complex-dependent MALT1 activation facilitates myocardial oxidative stress in doxorubicin-treated mice via enhancing k48-linked ubiquitination of Nrf2 | ||
Food Funct Allicin suppressed Escherichia coli-induced urinary tract infections by a novel MALT1/NF-κB pathway. | ||
Eur J Pharmacol Sonic hedgehog signaling facilitates pyroptosis in mouse heart following ischemia/reperfusion via enhancing the formation of CARD10-BCL10-MALT1 complex |
