MALT1 Polyclonal antibody

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  WB analysis of HeLa using 11660-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11660-1-AP (MALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11660-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11660-1-AP (MALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11660-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11660-1-AP (MALT1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11660-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MALT1 antibody (11660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HepG2 using 11660-1-AP

  Immunofluorescent analysis of HepG2 cells using 11660-1-AP (MALT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MALT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11660-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:3.4.22.-, MALT 1, MALT lymphoma-associated translocation, MLT, MLT1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

11660-1-AP targets MALT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2182

  Product name: Recombinant human MALT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 126-450 aa of BC030143

  Sequence: GIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRSVDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYMVPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIDELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLL

• Full Name: mucosa associated lymphoid tissue lymphoma translocation gene 1
• Calculated Molecular Weight: 824 aa, 92 kDa
• Observed Molecular Weight: 92-100 kDa
• GenBank Accession Number: BC030143
• Gene Symbol: MALT1
• Gene ID (NCBI): 10892
• RRID: AB_2139135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UDY8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Eur J Pharmacol; Inhibition of MALT1 reduces ferroptosis in rat hearts following ischemia/reperfusion via enhancing the Nrf2/SLC7A11 pathway; Authors - Ya-Qian Jiang
mouse,human	WB	ACS Chem Neurosci; Telaprevir Improves Memory and Cognition in Mice Suffering Ischemic Stroke via Targeting MALT1-Mediated Calcium Overload and Necroptosis; Authors - Yi-Yue Zhang
human	IHC	Biomedicines; Mucosa-Associated Lymphoid Tissue 1 Is an Oncogene Inducing Cell Proliferation, Invasion, and Tumor Growth via the Upregulation of NF-κB Activity in Human Prostate Carcinoma Cells.; Authors - Ke-Hung Tsui
rat	IHC	Food Funct; Allicin suppressed Escherichia coli-induced urinary tract infections by a novel MALT1/NF-κB pathway.; Authors - Zhenglin Chang
rat,mouse	WB	Arch Biochem Biophys; MALT1 promotes necroptosis in stroke rat brain via targeting the A20/RIPK3 pathway; Authors - Zi-Mei Peng
rat	WB,IF,CoIP	Mol Neurobiol; The Weakened Interaction Between HECTD4 and GluN2B in Ischemic Stroke Promotes Calcium Overload and Brain Injury Through a Mechanism Involving the Decrease of GluN2B and MALT1 Ubiquitination; Authors - Yi-Yue Zhang ; KD Validated