Validation Data Gallery
|Positive WB detected in||mouse cerebellum tissue, human brain tissue|
|Positive IHC detected in||mouse brain tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, mouse brain tissue|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21633-1-AP is MAP1B Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAP1B fusion protein Ag16255|
|Full Name||microtubule-associated protein 1B|
|Calculated molecular weight||2468 aa, 271 kDa|
|Observed molecular weight||320 kDa|
|GenBank accession number||BC141853|
|Gene ID (NCBI)||4131|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)
|Product Specific Protocols|
|WB protocol for MAP1B antibody 21633-1-AP||Download protocol|
|IHC protocol for MAP1B antibody 21633-1-AP||Download protocol|
|IF protocol for MAP1B antibody 21633-1-AP||Download protocol|
|FC protocol for MAP1B antibody 21633-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Neural Regen Res
GIT1 enhances neurite outgrowth by stimulating microtubule assembly.
Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.
eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.
MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Albane (Verified Customer) (02-18-2020)
A lot of bands, none at the correct size