Validation Data Gallery
|Positive WB detected in
|K-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells
|Positive IHC detected in
|human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|MAP2K4 fusion protein Ag11376
|mitogen-activated protein kinase kinase 4
|Calculated molecular weight
|399 aa, 44 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.
J Hazard Mater
Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.
J Cell Mol Med
NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.
Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.
Int J Biochem Cell Biol
Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.
Dig Dis Sci
LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.