MAP2K4 Polyclonal antibody

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  WB analysis using 17340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 17340-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17340-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17340-1-AP (MAP2K4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17340-1-AP (MAP2K4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17340-1-AP (MAP2K4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 17340-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17340-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

MAP2K4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17340-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below

Product Information

17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11376

  Product name: Recombinant human MAP2K4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 50-399 aa of BC036032

  Sequence: NPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD

• Full Name: mitogen-activated protein kinase kinase 4
• Calculated Molecular Weight: 399 aa, 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC036032
• Gene Symbol: MAP2K4
• Gene ID (NCBI): 6416
• RRID: AB_2878389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P45985
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu
mouse,rat	WB	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
mouse	WB	J Cell Mol Med; NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.; Authors - Ze-Qing Pu
rat	WB	Cells; Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.; Authors - Elaheh Soleimanpour
human	WB	Int J Biochem Cell Biol; Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.; Authors - Xuechao Wan ; KD Validated
human	WB	Dig Dis Sci; LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.; Authors - Jianhui Liu