Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20184-1-AP targets MAPK12 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14073 Product name: Recombinant human MAPK12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 216-349 aa of BC015741 Sequence: MAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHDTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVL Predict reactive species |
| Full Name | mitogen-activated protein kinase 12 |
| Calculated Molecular Weight | 367 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC015741 |
| Gene Symbol | MAPK12 |
| Gene ID (NCBI) | 6300 |
| RRID | AB_10665949 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53778 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAPK12 antibody 20184-1-AP | Download protocol |
| IP protocol for MAPK12 antibody 20184-1-AP | Download protocol |
| WB protocol for MAPK12 antibody 20184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAPK12 (p38γ) polyclonal antibody (Cat# 20184-1-AP) has 8 citations. These appear in journals including Nature Microbiology, Journal of Orthopaedic Translation, International Journal of Molecular Medicine, Inflammation and Regeneration, Biochemical Pharmacology, BMC Endocrine Disorders, Tumor Biology, and Clinical and Translational Oncology. Associated research fields span infectious disease (anthrax toxin), osteoarthritis and cellular senescence, acute liver injury, keloid fibrosis and glycolysis, urate reabsorption pharmacology, endocrinology (prolactinomas), esophageal squamous cell carcinoma, and diffuse large B-cell lymphoma.
| Species | Application | Title |
|---|---|---|
J Orthop Translat Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis | ||
Int J Mol Med Hepatic p38γ exacerbates acetaminophen‑induced acute liver injury via PI3K/Akt‑dependent mechanisms.
| ||
Inflamm Regen SPARC activates p38γ signaling to promote PFKFB3 protein stabilization and contributes to keloid fibroblast glycolysis | ||
Biochem Pharmacol Sodium butyrate upregulates URAT1-mediated urate reabsorption in HK2 cells by activating FFAR2/p38 MAPK/ELK1/ARRB2 axis and relieving NF-κB-HNF1β competitive binding. | ||
BMC Endocr Disord The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas. |
