MASP2 Polyclonal antibody

MASP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55252-1-AP

Synonyms

MAP19, MASP 2, MASP2, sMAP

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Validation Data Gallery

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  WB analysis using 55252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55252-1-AP (MASP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 55252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55252-1-AP (MASP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55252-1-AP (MASP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55252-1-AP targets MASP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mannan-binding lectin serine peptidase 2
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_006610
• Gene Symbol: MASP2
• Gene ID (NCBI): 10747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mannan-binding lectin serine protease 2 (MASP2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. MASP2 contains a 15-amino acid signal peptide, the 3 amino acids essential for the active center of a serine protease, and calcium-binding residues in the epidermal growth factor like domain.