MBTD1 Polyclonal antibody

MBTD1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26098-1-AP

Synonyms

mbt domain containing 1, MBTD1, SA49P01

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Validation Data Gallery

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  WB analysis of Jurkat using 26098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 26098-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 26098-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26098-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26098-1-AP (MBTD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 26098-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using MBTD1 antibody (26098-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, mouse spleen tissue, HL-60 cells
• Positive IF detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 26098-1-AP is MBTD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MBTD1 fusion protein Ag22059
• Full Name: mbt domain containing 1
• Calculated Molecular Weight: 628 aa, 70 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC101736
• Gene Symbol: MBTD1
• Gene ID (NCBI): 54799
• RRID: AB_2880374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Basis Dis; BAP18 acting as a novel peroxisome proliferator-activated receptor α co-regulator contributes to hepatocellular carcinoma progression; Authors - Wei Liu