MDC1 Polyclonal antibody

MDC1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24721-1-AP

Synonyms

KIAA0170, MDC1, NFBD1

---

Validation Data Gallery

• 

  IHC staining of human testis using 24721-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24721-1-AP (MDC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 24721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MDC1 antibody (24721-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

24721-1-AP targets MDC1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MDC1 fusion protein Ag20135
• Full Name: mediator of DNA damage checkpoint 1
• Calculated Molecular Weight: 2089 aa, 227 kDa
• GenBank Accession Number: BC152556
• Gene Symbol: MDC1
• Gene ID (NCBI): 9656
• RRID: AB_2879688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

Publications

Species	Application	Title
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao
mouse	IF	Cell Death Dis; Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma.; Authors - Yuanyuan Zhao
human	IF	Cancers (Basel); SFN Enhanced the Radiosensitivity of Cervical Cancer Cells via Activating LATS2 and Blocking Rad51/MDC1 Recruitment to DNA Damage Site.; Authors - Shiyu Wang
human	WB	DNA Repair (Amst); Functional defects of cancer-associated MDC1 mutations in DNA damage repair; Authors - Rong Xie