MDC1 Polyclonal antibody

MDC1 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24721-1-AP

Synonyms

KIAA0170, MDC1, NFBD1



Tested Applications

Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 24721-1-AP is MDC1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDC1 fusion protein Ag20135
Full Name mediator of DNA damage checkpoint 1
Calculated molecular weight 2089 aa, 227 kDa
GenBank accession numberBC152556
Gene symbol MDC1
Gene ID (NCBI) 9656
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

Protocols

Product Specific Protocols
IHC protocol for MDC1 antibody 24721-1-APDownload protocol
IF protocol for MDC1 antibody 24721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Death Dis

Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma.

Authors - Yuanyuan Zhao