MECP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MECP2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ChIP

Conjugate

Unconjugated

Publications

11

Cat no : 10861-1-AP

Synonyms

AUTSX3, MeCp 2 protein, MECP2, Methyl CpG binding protein 2, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 10861-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10861-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10861-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10861-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10861-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 10861-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 10861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10861-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10861-1-AP

  IP Result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with MCF-7 cells lysate 1280ug.

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  IP experiment of MCF-7 using 10861-1-AP

  IP Result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with MCF-7 cells lysate 1280ug.

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  IHC staining of human brain using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10861-1-AP (MECP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10861-1-AP (MECP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10861-1-AP

  Immunofluorescent analysis of HepG2 cells using 10861-1-AP (MECP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 10861-1-AP

  1X10^6 NIH/3T3 cells were stained with 0.2ug MECP2 antibody (10861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, SH-SY5Y cells, mouse heart tissue, mouse lung tissue, mouse ovary tissue, mouse brain tissue, human brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human brain tissue, human gliomas tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: NIH/3T3 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Product Information

10861-1-AP targets MECP2 in WB, IP, IHC, IF, FC, ChIP applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MECP2 fusion protein Ag1309
• Full Name: methyl CpG binding protein 2 (Rett syndrome)
• Calculated molecular weight: 52 kDa, 53 kDa
• Observed molecular weight: 75 kDa
• GenBank accession number: BC011612
• Gene symbol: MECP2
• Gene ID (NCBI): 4204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).

Publications

Species	Application	Title
mouse	WB	Int J Med Sci; MicroRNA-29a is a key regulon that regulates BRD4 and mitigates liver fibrosis in mice by inhibiting hepatic stellate cell activation.; Authors - Ying-Hsien Huang
human	WB, IF	Clin Immunol; Specificity of Antinuclear Autoantibodies Recognizing the Dense Fine Speckled Nuclear Pattern: Preferential Targeting of DFS70/LEDGFp75 Over its Interacting Partner MeCP2.; Authors - Anamika Basu
		Mol Med Rep; Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro.; Authors - Ning Song
human	WB	Clin Proteomics; Integration of transcriptome and proteome profiles in placenta accreta reveals trophoblast over-migration as the underlying pathogenesis.; Authors - Na Li ; KD Validated
rat	WB,IHC	Brain Res; Intrathecal 5-azacytidine inhibits global DNA methylation and methyl- CpG-binding protein 2 expression and alleviates neuropathic pain in rats following chronic constriction injury.; Authors - Wang Ying Y
	ChIP	Sci Rep; A prelude to the proximity interaction mapping of CXXC5.; Authors - Gamze Ayaz