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  • KD/KO Validated

MECP2 Polyclonal antibody

MECP2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10861-1-AP

Synonyms

MRX16, Methyl-CpG-binding protein 2, Methyl CpG binding protein 2, MeCp-2 protein, MeCp 2 protein



Tested Applications

Positive WB detected inMCF-7 cells, mouse lung tissue, mouse brain tissue, rat lung tissue
Positive IP detected inHEK-293T cells, MCF-7 cells
Positive IHC detected inhuman gliomas tissue, human breast cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10861-1-AP targets MECP2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MECP2 fusion protein Ag1309
Full Name methyl CpG binding protein 2 (Rett syndrome)
Calculated Molecular Weight 52 kDa, 53 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC011612
Gene Symbol MECP2
Gene ID (NCBI) 4204
RRIDAB_2143843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).

Protocols

Product Specific Protocols
WB protocol for MECP2 antibody 10861-1-APDownload protocol
IHC protocol for MECP2 antibody 10861-1-APDownload protocol
IF protocol for MECP2 antibody 10861-1-APDownload protocol
IP protocol for MECP2 antibody 10861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseChIP

Science

MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion.

Authors - Abdulkhaleg Ibrahim
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
  • KD Validated
humanWB, IF

Clin Immunol

Specificity of Antinuclear Autoantibodies Recognizing the Dense Fine Speckled Nuclear Pattern: Preferential Targeting of DFS70/LEDGFp75 Over its Interacting Partner MeCP2.

Authors - Anamika Basu
mouse,humanWB

Br J Pharmacol

Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.

Authors - Ai Wei
ratWB

Int J Biol Macromol

SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.

Authors - Ruike Wang
humanWB

Mol Cancer Res

The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity.

Authors - Leoh Lai Sum LS

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Sonam (Verified Customer) (05-31-2024)

Antibody works well for IHC and western blotting

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000(WB); 1:100 (IHC)
  • Cell Tissue Type: macrophages, cancer cell