Tested Applications
| Positive WB detected in | MCF-7 cells, mouse lung tissue, mouse brain tissue, rat lung tissue |
| Positive IP detected in | HEK-293T cells, MCF-7 cells |
| Positive IHC detected in | human gliomas tissue, human breast cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| ChIP | See 4 publications below |
Product Information
10861-1-AP targets MECP2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MECP2 fusion protein Ag1309 Predict reactive species |
| Full Name | methyl CpG binding protein 2 (Rett syndrome) |
| Calculated Molecular Weight | 52 kDa, 53 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC011612 |
| Gene Symbol | MECP2 |
| Gene ID (NCBI) | 4204 |
| RRID | AB_2143843 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51608 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IHC protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IF protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IP protocol for MECP2 antibody 10861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion. | ||
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
| ||
Clin Immunol Specificity of Antinuclear Autoantibodies Recognizing the Dense Fine Speckled Nuclear Pattern: Preferential Targeting of DFS70/LEDGFp75 Over its Interacting Partner MeCP2. | ||
Br J Pharmacol Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis. | ||
Int J Biol Macromol SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo. | ||
Mol Cancer Res The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sonam (Verified Customer) (05-31-2024) | Antibody works well for IHC and western blotting
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