• Featured Product
  • KD/KO Validated

CoraLite®555-conjugated MECP2 Polyclonal antibody

MECP2 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-10861

Synonyms

AUTSX3, DKFZp686A24160, MeCp 2 protein, MECP2, Methyl CpG binding protein 2, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL555-10861 targets MECP2 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MECP2 fusion protein Ag1309
Full Name methyl CpG binding protein 2 (Rett syndrome)
Calculated molecular weight 52 kDa, 53 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC011612
Gene symbol MECP2
Gene ID (NCBI) 4204
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths555 nm / 570 nm
Form Liquid
Purification Method Antigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).

Protocols

Product Specific Protocols
IF protocol for CL555 MECP2 antibody CL555-10861Download protocol
Standard Protocols
Click here to view our Standard Protocols