MED6 Monoclonal antibody

• 

  WB analysis using 68352-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68352-1-Ig (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

MED6 Monoclonal Antibody for WB, ELISA

Cat No. 68352-1-Ig

Clone No.3E4G10

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28

---

Tested Applications

• Positive WB detected in: U2OS cells, LNCaP cells, HeLa cells, T-47D cells, SK-BR-3 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68352-1-Ig targets MED6 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7645

  Product name: Recombinant human MED6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-246 aa of BC004106

  Sequence: MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKFPPKFVQLKPGEKPVPVDQTKKEAEPIPETVKPEEKETTKNVQQTVSAKGPPEKRMRLQ

• Full Name: mediator complex subunit 6
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004106
• Gene Symbol: MED6
• Gene ID (NCBI): 10001
• RRID: AB_3085075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75586
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.