METTL2 Polyclonal antibody, PBS Only

• 

  WB analysis using 16983-1-AP (same clone as 16983-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16983-1-AP (METTL2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 16983-1-AP (same clone as 16983-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (16983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

METTL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16983-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

16983-1-PBS targets METTL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10410

  Product name: Recombinant human METTL2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-313 aa of BC107586

  Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS

• Full Name: methyltransferase like 2B
• Calculated Molecular Weight: 313 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC107586
• Gene Symbol: METTL2B
• Gene ID (NCBI): 55798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P1Q9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.