Tested Applications
| Positive WB detected in | SH-SY5Y cells, MCF-7 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16983-1-AP targets METTL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10410 Product name: Recombinant human METTL2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC107586 Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS Predict reactive species |
| Full Name | methyltransferase like 2B |
| Calculated Molecular Weight | 313 aa, 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC107586 |
| Gene Symbol | METTL2B |
| Gene ID (NCBI) | 55798 |
| RRID | AB_2923564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P1Q9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL2 antibody 16983-1-AP | Download protocol |
| IHC protocol for METTL2 antibody 16983-1-AP | Download protocol |
| WB protocol for METTL2 antibody 16983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
METTL2 Polyclonal antibody (Cat# 16983-1-AP) has 6 citations published in Nature Communications, Stem Cell Research & Therapy, Journal of Cellular and Molecular Medicine, Journal of Inflammation Research, Brain Research Bulletin, and BMC Cancer. Associated research fields include tRNA modification and mRNA translation, osteogenic differentiation, breast cancer prognosis, osteoarthritis diagnosis, spinal cord injury, and ovarian cancer progression.
| Species | Application | Title |
|---|---|---|
Nat Commun m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response
| ||
Stem Cell Res Ther Mechanical tension-induced Dalrd3 elevation enhances osteogenic differentiation of bone suture stem cells by upregulating Id3 translation | ||
J Cell Mol Med Nomogram Based on A-To-I RNA Editing for Predicting Overall Survival in Patients With Breast Cancer | ||
J Inflamm Res Development and Validation of Diagnostic Models for Transcriptomic Signature Genes for Multiple Tissues in Osteoarthritis | ||
Brain Res Bull Rab3a attenuates spinal cord injury by mediating vesicle release
| ||
BMC Cancer METTL2B m3C RNA transferase: oncogenic role in ovarian cancer progression via regulation of the mTOR/AKT pathway and its link to the tumor immune microenvironment |
