METTL2 Polyclonal antibody

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  WB analysis using 16983-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16983-1-AP (METTL2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 16983-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 16983-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 16983-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (16983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

METTL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16983-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

methyltransferase like 2B, METL, METTL2, METTL2A, METTL2A/B, METTL2B, PSENIP1

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, MCF-7 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

16983-1-AP targets METTL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10410

  Product name: Recombinant human METTL2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-313 aa of BC107586

  Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS

• Full Name: methyltransferase like 2B
• Calculated Molecular Weight: 313 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC107586
• Gene Symbol: METTL2B
• Gene ID (NCBI): 55798
• RRID: AB_2923564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P1Q9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response; Authors - Jia Cui ; KO Validated
human	IHC	J Inflamm Res; Development and Validation of Diagnostic Models for Transcriptomic Signature Genes for Multiple Tissues in Osteoarthritis; Authors - Qichang Gao
rat	WB	Brain Res Bull; Rab3a attenuates spinal cord injury by mediating vesicle release; Authors - Yuhao Yang ; KD Validated
human	WB,IHC	BMC Cancer; METTL2B m3C RNA transferase: oncogenic role in ovarian cancer progression via regulation of the mTOR/AKT pathway and its link to the tumor immune microenvironment; Authors - Yizi Meng