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METTL3 Polyclonal antibody, PBS Only (29314-1-PBS)

Filter: AllWBIHC

WB analysis using 29314-1-AP (same clone as 29314-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.
WB analysis using 29314-1-AP (same clone as 29314-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 29314-1-AP (same clone as 29314-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 29314-1-AP (same clone as 29314-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 29314-1-AP (same clone as 29314-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 29314-1-AP (same clone as 29314-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 29314-1-AP (same clone as 29314-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 29314-1-AP (same clone as 29314-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.

METTL3 Polyclonal antibody, PBS Only

Name: METTL3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29314-1-PBS

METTL3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29314-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29314-1-PBS targets METTL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag30638 Product name: Recombinant human METTL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 285-485 aa of BC001650 Sequence: MKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLV Predict reactive species
Full Name	methyltransferase like 3
Calculated Molecular Weight	64 kDa
Observed Molecular Weight	65-70 kDa
GenBank Accession Number	BC001650
Gene Symbol	METTL3
Gene ID (NCBI)	56339
RRID	AB_3086114
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86U44
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.