MFN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MFN2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

362

Reviews (4)

Cat no : 12186-1-AP

Synonyms

mitofusin 2, MARF, KIAA0214, HSG, EC:3.6.5.-

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Validation Data Gallery

• 

  WB analysis using 12186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12186-1-AP

  WB result of MFN2 antibody (12186-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells.

• 

  WB analysis using 12186-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12186-1-AP

  IP result of anti-MFN2 (IP:12186-1-AP, 4ug; Detection:12186-1-AP 1:10000) with mouse kidney tissue lysate 2160 ug.

• 

  IHC staining of human colon cancer using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, mouse kidney tissue, rat brain tissue, rat heart tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 16 publications below
• WB: See 49 publications below
• IHC: See 23 publications below
• IF: See 28 publications below
• IP: See 7 publications below
• CoIP: See 4 publications below

Product Information

12186-1-AP targets MFN2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, zebrafish, bovine, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFN2 fusion protein Ag2845
• Full Name: mitofusin 2
• Calculated Molecular Weight: 757 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC017061
• Gene Symbol: MFN2
• Gene ID (NCBI): 9927
• RRID: AB_2266320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

Publications

Species	Application	Title
mouse		Cell; Mitocytosis, a migrasome-mediated mitochondrial quality-control process.; Authors - Haifeng Jiao ; KD Validated
mouse		Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
mouse		ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse		Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
mouse		Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human		Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (09-23-2024)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

MFN2 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:12186-1-AP)

FH

Nin (Verified Customer) (02-27-2023)

A reliable Ab to detect endogenous Mfn2

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

MFN2 Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:12186-1-AP)

FH

Mi (Verified Customer) (02-21-2023)

This antibody works pretty well in adipocytes, we got a single and clean band at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Julia (Verified Customer) (11-16-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293 cells

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