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  • KD/KO Validated

MFN2 Polyclonal antibody

MFN2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12186-1-AP

Synonyms

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, Mitofusin 2, Transmembrane GTPase MFN2



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, COLO 320 cells, mouse kidney tissue, mouse liver tissue, rat brain tissue, rat kidney tissue, rat liver tissue, rat heart tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12186-1-AP targets MFN2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, chicken, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFN2 fusion protein Ag2845
Full Name mitofusin 2
Calculated molecular weight 757 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC017061
Gene symbol MFN2
Gene ID (NCBI) 9927
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

Protocols

Product Specific Protocols
WB protocol for MFN2 antibody 12186-1-APDownload protocol
IHC protocol for MFN2 antibody 12186-1-APDownload protocol
IP protocol for MFN2 antibody 12186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Mitocytosis, a migrasome-mediated mitochondrial quality-control process.

Authors - Haifeng Jiao
  • KD Validated
mouseWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
mouseWB

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
WB

Nat Commun

Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.

Authors - Huan Yang
mouseWB

Nat Commun

PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.

Authors - Ling Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Julia (Verified Customer) (11-16-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293 cells