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MFN2 Monoclonal antibody
MFN2 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IHC
Conjugate
Unconjugated
CloneNo.
5F3B3
Cat no : 67487-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
67487-1-Ig targets MFN2 in WB, IHC applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, fish |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | MFN2 fusion protein Ag29873 |
Full Name | mitofusin 2 |
Calculated molecular weight | 757 aa, 86 kDa |
Observed molecular weight | 86 kDa |
GenBank accession number | BC017061 |
Gene symbol | MFN2 |
Gene ID (NCBI) | 9927 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.
Protocols
Product Specific Protocols | |
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WB protocol for MFN2 antibody 67487-1-Ig | Download protocol |
IHC protocol for MFN2 antibody 67487-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Physiol Sci Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart | ||
Molecules Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy. |