MFN2 Monoclonal antibody

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• KD/KO Validated

MFN2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

CloneNo.

5F3B3

Cat no : 67487-1-Ig

Synonyms

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2

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Validation Data Gallery

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  WB analysis of HeLa using 67487-1-Ig

  WB result of MFN2 antibody (67487-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells.

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  WB analysis using 67487-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67487-1-Ig (MFN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 67487-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 67487-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67487-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN2 antibody (67487-1-Ig, Clone: 5F3B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 9 publications below
• IHC: See 3 publications below

Product Information

67487-1-Ig targets MFN2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat, fish
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MFN2 fusion protein Ag29873
• Full Name: mitofusin 2
• Calculated molecular weight: 757 aa, 86 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC017061
• Gene symbol: MFN2
• Gene ID (NCBI): 9927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

Publications

Species	Application	Title
human	WB	Molecules; Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.; Authors - Jiling Feng
fish	WB	J Physiol Sci; Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart; Authors - Zhanglin Chen
rat	IHC	J Mol Histol; Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats; Authors - Fan Wang
rat	WB,IHC	Nutrients; Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats; Authors - Yujie Zhong
human	WB	Autophagy; Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1; Authors - Ying Liu
mouse	WB	Biomolecules; Multi-Targeting Intranasal Nanoformulation as a Therapeutic for Alzheimer's Disease; Authors - Oksana Fihurka