CTAGE5 Polyclonal antibody

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  WB analysis using 31986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31986-1-AP (CTAGE5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

CTAGE5 Polyclonal Antibody for WB, ELISA

Cat No. 31986-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

cTAGE family member 5, CTAGE family member 5 ER export factor, CTAGE family, member 5, Cutaneous T-cell lymphoma-associated antigen 5, MEA11

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Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31986-1-AP targets CTAGE5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34060

  Product name: Recombinant human MIA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 32-376 aa of BC130537

  Sequence: CGDLECEALINRVSAMRDYRGPDCRYLNFTKGEEISVYVKLAGEREDLWAGSKGKEFGYFPRDAVQIEEVFISEEIQMSTKESDFLCLLGVSYTFDNEDSELNGDYGENIYPYEEDKDEKSSIYESDFQIEPGFYATYESTLFEDQVPALEAPEDIGSTSESKDWEEVVVESMEQDRIPEVHVPPSSAVSGVKEWFGLGGEQAEEKAFESVIEPVQESSFRSRKIAVEDENDLEELNNGEPQTEHQQESESEIDSVPKTQSELASESEHIPKPQSTGWFGGGFTSYLGFGDEDTGLELIAEESNPPLQDFPNSISSDKEATVPCTEILTEKKDTITNDSLSLKPS

• Full Name: CTAGE family, member 5
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: BC130537
• Gene Symbol: CTAGE5
• Gene ID (NCBI): 4253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96PC5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cutaneous T-cell lymphoma-associated antigen 5 (CTAGE5, also known as MIA2) is a protein involved in the regulation of intracellular transport and secretion. It plays a crucial role in the trafficking of large cargo proteins, such as collagen VII, from the endoplasmic reticulum (ER) to the Golgi apparatus (PMID: 25202031; 27170179). CTAGE5 forms a complex with TANGO1, another cargo receptor, to facilitate the export of collagen VII from the ER (PMID: 21525241). This process is essential for the proper secretion of collagen and other large proteins, which are critical for maintaining tissue integrity and function. Additionally, CTAGE5 produces 240 and 120 kDa protein isoforms (PMID: 21807889).