MIA3 Polyclonal antibody, PBS Only

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  WB analysis of HEK-293T using 17481-1-AP (same clone as 17481-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 17481-1-AP (MIA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17481-1-AP (same clone as 17481-1-PBS)

  IP result of anti-MIA3 (IP:17481-1-AP, 5ug; Detection:17481-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MIA3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 17481-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

17481-1-PBS targets MIA3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11514

  Product name: Recombinant human MIA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-467 aa of BC047116

  Sequence: KAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRCPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGEPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

• Full Name: melanoma inhibitory activity family, member 3
• Calculated Molecular Weight: 214 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC047116
• Gene Symbol: MIA3
• Gene ID (NCBI): 375056
• RRID: AB_2878411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5JRA6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.