MIA3 Polyclonal antibody

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  WB analysis of HEK-293T using 17481-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 17481-1-AP (MIA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17481-1-AP

  IP result of anti-MIA3 (IP:17481-1-AP, 5ug; Detection:17481-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of mouse brain using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

MIA3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17481-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

ARNT, C219 reactive peptide, D320, KIAA0268, MIA3, TANGO, TANGO1, UNQ6077

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

17481-1-AP targets MIA3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11514

  Product name: Recombinant human MIA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-467 aa of BC047116

  Sequence: KAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRCPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGEPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

• Full Name: melanoma inhibitory activity family, member 3
• Calculated Molecular Weight: 214 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC047116
• Gene Symbol: MIA3
• Gene ID (NCBI): 375056
• RRID: AB_2878411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5JRA6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Front Endocrinol (Lausanne); MIA SH3 Domain ER Export Factor 3 Deficiency Prevents Neointimal Formation by Restoring BAT-Like PVAT and Decreasing VSMC Proliferation and Migration.; Authors - Yu Lei ; KD Validated
human	WB,IF	Arthritis Rheumatol; Caspase-1 Enhances TANGO1 Expression to Promote Procollagen Export from the Endoplasmic Reticulum in Systemic Sclerosis Contributing to Fibrosis; Authors - Lianne M Connolly ; KD Validated
zebrafish	IF	Elife; Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset; Authors - Arian Mansur
human	WB,IHC,IF,CoIP	Mol Cell Biochem; MIA3 promotes the degradation of GSH (glutathione) by binding to CHAC1, thereby promoting the progression of hepatocellular carcinoma; Authors - Zhou Wanbiao ; KD Validated
		Dev Cell; Endoplasmic reticulum exit sites are segregated for secretion based on cargo size; Authors - Sonashree Saxena