Mouse MIF Polyclonal antibody, PBS Only

• 

  WB analysis using 30957-1-AP (same clone as 30957-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30957-1-AP (MIF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 30957-1-AP (same clone as 30957-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using MIF antibody (30957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 30957-1-AP (same clone as 30957-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MIF antibody (30957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

• 

  FC experiment of NIH/3T3 using 30957-1-AP (same clone as 30957-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Mouse MIF (30957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

MIF Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 30957-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

GIF

---

Product Information

30957-1-PBS targets MIF in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: macrophage migration inhibitory factor
• Calculated Molecular Weight: 13KD
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: NM_010798.2
• Gene Symbol: Mif
• Gene ID (NCBI): 17319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P34884
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.