Melan-A Polyclonal antibody

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  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (Melan-A antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (Melan-A antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

Melan-A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18472-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

melan A, Melan-A , MLANA, Protein Melan A, Protein Melan-A, Melanoma antigen recognized by T-cells 1, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA, Antigen SK29-AA, MART 1, MART1, MART-1

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Tested Applications

• Positive WB detected in: mouse eye tissue
• Positive IHC detected in: human malignant melanoma tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

18472-1-AP targets Melan-A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13346

  Product name: Recombinant human MLANA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-118 aa of BC014423

  Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

• Full Name: melan-A
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13-20 kDa
• GenBank Accession Number: BC014423
• Gene Symbol: MelanA
• Gene ID (NCBI): 2315
• RRID: AB_2878545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16655
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Int J Mol Sci; The Role of Hedgehog Signaling in the Melanoma Tumor Bone Microenvironment; Authors - Karnoon Shamsoon
mouse	WB	Life Sci; Differentiation-inducing factor-1 reduces lipopolysaccharide-induced vascular cell adhesion molecule-1 by suppressing mTORC1-S6K signaling in vascular endothelial cells; Authors - Masaki Arioka
mouse	IHC	Cell Death Discov; Unique lipid composition maintained by extracellular blockade leads to prooncogenicity; Authors - Kai Kudo
mouse	IF	Mol Ther Nucleic Acids; IL-12 and PD-1 peptide combination gene therapy for the treatment of melanoma; Authors - Loree C Heller
human	IF	Biochim Biophys Acta Mol Cell Res; MitoQ for vitiligo by mitigating PARP1 translocation aberrations: Network pharmacology and experimental validation; Authors - Renxue Xiong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chromogenic Kit from Leica Antigen Retrival ER1 (ph6) for 30 min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Melanoma Tuor

Melan-A Antibody Immunohistochemistry validation (1:500 dilution) in Melanoma Tuor (Cat no:18472-1-AP)