Melan-A Polyclonal antibody

Melan-A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 18472-1-AP


Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, MLANA, Protein Melan A

Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman malignant melanoma tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18472-1-AP is Melan-A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Melan-A fusion protein Ag13346
Full Name melan-A
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13-20 kDa
GenBank Accession NumberBC014423
Gene Symbol MLANA
Gene ID (NCBI) 2315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)


Product Specific Protocols
WB protocol for Melan-A antibody 18472-1-APDownload protocol
IHC protocol for Melan-A antibody 18472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Mol Sci

The Role of Hedgehog Signaling in the Melanoma Tumor Bone Microenvironment

Authors - Karnoon Shamsoon

Life Sci

Differentiation-inducing factor-1 reduces lipopolysaccharide-induced vascular cell adhesion molecule-1 by suppressing mTORC1-S6K signaling in vascular endothelial cells

Authors - Masaki Arioka


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Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chromogenic Kit from Leica Antigen Retrival ER1 (ph6) for 30 min

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Melanoma Tuor
Melan-A Antibody Immunohistochemistry validation (1:500 dilution) in Melanoma Tuor (Cat no:18472-1-AP)