Melan-A Polyclonal antibody
Melan-A Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
Cat no : 18472-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse eye tissue |
Positive IHC detected in | human malignant melanoma tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 18472-1-AP is Melan-A Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Melan-A fusion protein Ag13346 |
Full Name | melan-A |
Calculated molecular weight | 13 kDa |
Observed molecular weight | 13-20 kDa |
GenBank accession number | BC014423 |
Gene symbol | MLANA |
Gene ID (NCBI) | 2315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)
Protocols
Product Specific Protocols | |
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WB protocol for Melan-A antibody 18472-1-AP | Download protocol |
IHC protocol for Melan-A antibody 18472-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |