Melan-A Polyclonal antibody

Melan-A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18472-1-AP

Synonyms

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, MLANA, Protein Melan A



Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman malignant melanoma tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18472-1-AP is Melan-A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Melan-A fusion protein Ag13346
Full Name melan-A
Calculated molecular weight 13 kDa
Observed molecular weight 13-20 kDa
GenBank accession numberBC014423
Gene symbol MLANA
Gene ID (NCBI) 2315
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)

Protocols

Product Specific Protocols
WB protocol for Melan-A antibody 18472-1-APDownload protocol
IHC protocol for Melan-A antibody 18472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols