Tested Applications
| Positive WB detected in | SK-MEL-30 cells, mouse eye tissue |
| Positive IHC detected in | human malignant melanoma tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SK-MEL-28 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18472-1-AP targets Melan-A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13346 Product name: Recombinant human MLANA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-118 aa of BC014423 Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP Predict reactive species |
| Full Name | melan-A |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 13-20 kDa |
| GenBank Accession Number | BC014423 |
| Gene Symbol | MelanA |
| Gene ID (NCBI) | 2315 |
| RRID | AB_2878545 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Melan-A antibody 18472-1-AP | Download protocol |
| IHC protocol for Melan-A antibody 18472-1-AP | Download protocol |
| WB protocol for Melan-A antibody 18472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Melan-A Polyclonal antibody (Cat# 18472-1-AP) has 8 citations. These appear in journals including Cell Death Discovery, Molecular Therapy Nucleic Acids, Life Sciences, International Journal of Molecular Sciences, HLA, iScience, BBA Molecular Cell Research, and Gene Therapy. Research fields span melanoma biology and metastasis, lipid-mediated prooncogenicity, gene therapy, vascular endothelial signaling, Hedgehog signaling in the tumor microenvironment, HLA-related immunology, and vitiligo. Applications cited include IHC, IF, and WB in human and mouse models.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Unique lipid composition maintained by extracellular blockade leads to prooncogenicity | ||
Mol Ther Nucleic Acids IL-12 and PD-1 peptide combination gene therapy for the treatment of melanoma | ||
Life Sci Differentiation-inducing factor-1 reduces lipopolysaccharide-induced vascular cell adhesion molecule-1 by suppressing mTORC1-S6K signaling in vascular endothelial cells | ||
HLA Escape From Synthetic T Cell Activator Tebentafusp by Genomic HLA Loss: A Case Report. | ||
iScience Matched sampling reveals uncoupled phenotypic plasticity during metastasis formation in uveal melanoma. |
Reviews
What customers say
The antibody received a 5-star rating for use in immunohistochemistry on melanoma tumor tissue. The reviewer used a 1:500 dilution with a Leica Bond Rxm platform, Leica Red Chromogenic Kit, and ER1 (pH 6) antigen retrieval for 30 minutes, reporting satisfactory results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Federica (Verified Customer) (12-08-2023) | Leica Bond Rxm Red Chromogenic Kit from Leica Antigen Retrival ER1 (ph6) for 30 min
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