Melan-A Polyclonal antibody

Melan-A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18472-1-AP

Synonyms

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, MLANA, Protein Melan A

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Validation Data Gallery

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  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 18472-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18472-1-AP (MLANA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA antibody) at dilution of 1:4000 (under 40x lens).

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  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 18472-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18472-1-AP (MLANA Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse eye tissue
• Positive IHC detected in: human malignant melanoma tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18472-1-AP is Melan-A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Melan-A fusion protein Ag13346
• Full Name: melan-A
• Calculated molecular weight: 13 kDa
• Observed molecular weight: 13-20 kDa
• GenBank accession number: BC014423
• Gene symbol: MLANA
• Gene ID (NCBI): 2315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melan-A is a palmitoylated integral membrane protein of 118 amino acids with a short amino-terminal luminal domain and a longer carboxy-terminal cytoplasmic domain . The protein does not possess any detectable enzymatic activity and has not been linked to any of the numerous genetic defects that affect skin pigmentation. Melan-A is new immunohistochemical markers that can be used in the diagnosis of melanocytic lesions. (PMID: 15703212, PMID: 17445277)