MLK3 Polyclonal antibody, PBS Only

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  WB analysis using 11996-1-AP (same clone as 11996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11996-1-AP (MLK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11996-1-AP (same clone as 11996-1-PBS)

  WB result of MLK3 antibody (11996-1-AP, 1:500) with si-Control and si-MLK3 transfected HEK293 cells. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 11996-1-AP (same clone as 11996-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLK3 antibody (11996-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11996-1-AP (same clone as 11996-1-PBS)

  Immunofluorescent analysis of Hela cells, using MAP3K11 antibody 11996-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11996-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

11996-1-PBS targets MLK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2636

  Product name: Recombinant human MAP3K11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-379 aa of BC011263

  Sequence: MEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDFASILQQLEAL

• Full Name: mitogen-activated protein kinase kinase kinase 11
• Calculated Molecular Weight: 847 aa, 93 kDa
• Observed Molecular Weight: 93 and 65 kDa
• GenBank Accession Number: BC011263
• Gene Symbol: MLK3
• Gene ID (NCBI): 4296
• RRID: AB_10694137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16584
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).