Tested Applications
| Positive WB detected in | L02 cells, HEK-293 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11996-1-AP targets MLK3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2636 Product name: Recombinant human MAP3K11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-379 aa of BC011263 Sequence: MEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDFASILQQLEAL Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 11 |
| Calculated Molecular Weight | 847 aa, 93 kDa |
| Observed Molecular Weight | 93 and 65 kDa |
| GenBank Accession Number | BC011263 |
| Gene Symbol | MLK3 |
| Gene ID (NCBI) | 4296 |
| RRID | AB_10694137 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16584 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MLK3 antibody 11996-1-AP | Download protocol |
| IHC protocol for MLK3 antibody 11996-1-AP | Download protocol |
| WB protocol for MLK3 antibody 11996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MLK3 antibody (Cat# 11996-1-AP) has 12 citations published in journals including Metabolism, Cell Death & Disease, Acta Pharmacologica Sinica, International Journal of Molecular Medicine, Journal of Ethnopharmacology, Biochemical Pharmacology, Inflammation Research, Journal of Cellular and Molecular Medicine, Frontiers in Physiology, Journal of Virology, Journal of Bone and Mineral Metabolism, and Journal of Molecular Histology. Research fields span cardiac fibrosis, diabetes, osteoporosis, atherosclerosis, osteoarthritis, ferroptosis, pyroptosis, viral infection, and lung injury.
| Species | Application | Title |
|---|---|---|
Metabolism Setmelanotide alleviates diabetes-associated cognitive impairment by interrupting the binding of ICAM-1 and JIP1 in endothelial cells of the blood-brain barrier. | ||
Cell Death Dis Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload. | ||
Acta Pharmacol Sin Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy. | ||
Int J Mol Med Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway. | ||
J Ethnopharmacol Xinyang Tablet inhibits MLK3-mediated pyroptosis to attenuate inflammation and cardiac dysfunction in pressure overload. | ||
Biochem Pharmacol 2'-O-Galloylhyperin attenuates osteoclastogenesis and estrogen-deficiency osteoporosis by targeting MLK3-mediated NF-κB signaling. |
