|Positive WB detected in||HeLa cells, 4T1 cells, HepG2 cells, HUVEC cells|
|Positive IHC detected in||human liver cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66675-1-Ig targets MLKL in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MLKL fusion protein Ag15188|
|Full Name||mixed lineage kinase domain-like|
|Calculated molecular weight||471 aa, 54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC028141|
|Gene ID (NCBI)||197259|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291)
A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation.
Cell Death Differ
The bromodomain protein BRD4 positively regulates necroptosis via modulating MLKL expression.
J Cell Sci
FKBP12 mediates necroptosis by initiating RIPK1/RIPK3/MLKL signal transduction in response to TNF receptor 1 ligation.
Front Cell Dev Biol
TRADD Mediates RIPK1-Independent Necroptosis Induced by Tumor Necrosis Factor.
Acta Pharm Sin B
A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Fan (Verified Customer) (03-22-2021)
Mouse retinal tissue was used. Multiple bands were detected. The target band has the strongest intensity.
Paramananda (Verified Customer) (01-31-2019)