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  • KD/KO Validated

MLKL Monoclonal antibody

MLKL Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D4C6

Cat no : 66675-1-Ig

Synonyms

FLJ34389, MLKL



Tested Applications

Positive WB detected inHT-29 cells, 4T1 cells, PC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66675-1-Ig targets MLKL in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MLKL fusion protein Ag15188
Full Name mixed lineage kinase domain-like
Calculated molecular weight 471 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC028141
Gene symbol MLKL
Gene ID (NCBI) 197259
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291)

Protocols

Product Specific Protocols
WB protocol for MLKL antibody 66675-1-IgDownload protocol
IHC protocol for MLKL antibody 66675-1-IgDownload protocol
IF protocol for MLKL antibody 66675-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Adv Sci (Weinh)

Pressure Drives Rapid Burst-Like Coordinated Cellular Motion from 3D Cancer Aggregates.

Authors - Swetha Raghuraman
mouse

Cell Mol Life Sci

Thioredoxin reductase 3 suppression promotes colitis and carcinogenesis via activating pyroptosis and necrosis.

Authors - Qi Liu
mouseWB,IF

J Cell Physiol

L-OPA1 deficiency aggravates necroptosis of alveolar epithelial cells through impairing mitochondrial function during acute lung injury in mice.

Authors - Hui-Ling Jiang
ratWB

Front Cell Dev Biol

Adenosine Kinase Inhibition Prevents Severe Acute Pancreatitis via Suppressing Inflammation and Acinar Cell Necroptosis.

Authors - Shukun Sun
mouseWB

Front Cell Dev Biol

4-Hydroxy-2-Nonenal Promotes Cardiomyocyte Necroptosis via Stabilizing Receptor-Interacting Serine/Threonine-Protein Kinase 1.

Authors - Xiaoxuan Zhai
mouseWB

FASEB J

Serpina3c deficiency induced necroptosis promotes non-alcoholic fatty liver disease through β-catenin/Foxo1/TLR4 signaling.

Authors - Ling Lin Qian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Multiple bands were detected. The target band has the strongest intensity.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
MLKL Antibody Western Blot validation (1:500 dilution) in (Cat no:66675-1-Ig)
FH

Paramananda (Verified Customer) (01-31-2019)

Excellent antibody!

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Cornea