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  • KD/KO Validated

MLKL Monoclonal antibody

MLKL Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse and More (3)







Cat no : 66675-1-Ig


FLJ34389, MLKL

Tested Applications

Positive WB detected inHT-29 cells, 4T1 cells, PC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, mMSCs cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66675-1-Ig targets MLKL in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MLKL fusion protein Ag15188
Full Name mixed lineage kinase domain-like
Calculated Molecular Weight 471 aa, 54 kDa
Observed Molecular Weight35-40 kDa, 50-55 kDa
GenBank Accession NumberBC028141
Gene Symbol MLKL
Gene ID (NCBI) 197259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. The 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer, and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected as a 35kDa truncated isoform (PMID: 35965541).


Product Specific Protocols
WB protocol for MLKL antibody 66675-1-IgDownload protocol
IHC protocol for MLKL antibody 66675-1-IgDownload protocol
IF protocol for MLKL antibody 66675-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

Pressure Drives Rapid Burst-Like Coordinated Cellular Motion from 3D Cancer Aggregates.

Authors - Swetha Raghuraman

Acta Pharm Sin B

Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy

Authors - Jing Zhu

Acta Pharm Sin B

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.

Authors - Ziqiao Yuan

Aging Dis

MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.

Authors - Yanhui Cui

Cell Death Discov

Repositioning linifanib as a potent anti-necroptosis agent for sepsis

Authors - Liang Yu

Cell Mol Life Sci

Thioredoxin reductase 3 suppression promotes colitis and carcinogenesis via activating pyroptosis and necrosis.

Authors - Qi Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Multiple bands were detected. The target band has the strongest intensity.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
MLKL Antibody Western Blot validation (1:500 dilution) in (Cat no:66675-1-Ig)

Paramananda (Verified Customer) (01-31-2019)

Excellent antibody!

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Cornea