Validation Data Gallery
|Positive WB detected in||HT-29 cells, 4T1 cells, PC-3 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, mMSCs cells|
|Positive IHC detected in||human liver cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66675-1-Ig targets MLKL in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MLKL fusion protein Ag15188|
|Full Name||mixed lineage kinase domain-like|
|Calculated molecular weight||471 aa, 54 kDa|
|Observed molecular weight||35-40kDa, 50-55 kDa|
|GenBank accession number||BC028141|
|Gene ID (NCBI)||197259|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a prograwmmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected a 35kDa truncated isoform (PMID: 35965541).
Adv Sci (Weinh)
Pressure Drives Rapid Burst-Like Coordinated Cellular Motion from 3D Cancer Aggregates.
Acta Pharm Sin B
Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy
Acta Pharm Sin B
A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.
MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.
Cell Death Discov
Repositioning linifanib as a potent anti-necroptosis agent for sepsis
Cell Mol Life Sci
Thioredoxin reductase 3 suppression promotes colitis and carcinogenesis via activating pyroptosis and necrosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Fan (Verified Customer) (03-22-2021)
Mouse retinal tissue was used. Multiple bands were detected. The target band has the strongest intensity.
Paramananda (Verified Customer) (01-31-2019)