MLKL Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MLKL Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D4C6

Cat no : 66675-1-PBS

Synonyms

FLJ34389, MLKL

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Validation Data Gallery

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  WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66675-1-Ig (same clone as 66675-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66675-1-Ig (MLKL antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66675-1-PBS targets MLKL in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLKL fusion protein Ag15188
• Full Name: mixed lineage kinase domain-like
• Calculated Molecular Weight: 471 aa, 54 kDa
• Observed Molecular Weight: 35-40kDa, 50-55 kDa
• GenBank Accession Number: BC028141
• Gene Symbol: MLKL
• Gene ID (NCBI): 197259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a prograwmmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected a 35kDa truncated isoform (PMID: 35965541).