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- KD/KO Validated
CoraLite® Plus 488-conjugated MLKL Monoclonal antibody
MLKL Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3D4C6
Cat no : CL488-66675
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human liver cancer tissue |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
FC | See 1 publications below |
Product Information
CL488-66675 targets MLKL in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MLKL fusion protein Ag15188 |
Full Name | mixed lineage kinase domain-like |
Calculated Molecular Weight | 471 aa, 54 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | BC028141 |
Gene Symbol | MLKL |
Gene ID (NCBI) | 197259 |
RRID | AB_2883366 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 MLKL antibody CL488-66675 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Transl Med TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury |