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CoraLite®488-conjugated MLKL Monoclonal antibody

MLKL Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3D4C6

Cat no : CL488-66675

Synonyms

FLJ34389, MLKL



Tested Applications

Positive IF detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66675-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66675 targets MLKL in IF applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MLKL fusion protein Ag15188
Full Name mixed lineage kinase domain-like
Calculated molecular weight 471 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC028141
Gene symbol MLKL
Gene ID (NCBI) 197259
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Protocols

Product Specific Protocols
IF protocol for CL488 MLKL antibody CL488-66675Download protocol
Standard Protocols
Click here to view our Standard Protocols