MLPH Polyclonal antibody, PBS Only

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  WB analysis using 10338-1-AP (same clone as 10338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10338-1-AP (MLPH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IP experiment of A375 using 10338-1-AP (same clone as 10338-1-PBS)

  IP result of anti-Melanophilin (IP:10338-1-AP, 4ug; Detection:10338-1-AP 1:600) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10338-1-AP (same clone as 10338-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Melanophilin antibody (10338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MLPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10338-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A

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Product Information

10338-1-PBS targets MLPH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0316

  Product name: Recombinant human MLPH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 8-236 aa of BC001653

  Sequence: SKLTDEEAQHVLEVVQRDFDLRRKEEERLEALKGKIKKESSKRELLSDTAHLNETHCARCLQPYQLLVNSKRQCLECGLFTCKSCGRVHPEEQGWICDPCHLARVVKIGSLEWYYEHVKARFKRFGSAKVIRSLHGRLQGGAGPELISEERSGDSDQTDEDGEPGSEAQAQAQPFGSKKKRLLSVHDFDFEGDSDDSTQPQGHSLHLSSVPEARDSPQSLTDESCSEKA

• Full Name: melanophilin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa, 80 kDa
• GenBank Accession Number: BC001653
• Gene Symbol: MLPH
• Gene ID (NCBI): 79083
• RRID: AB_2146104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BV36
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.