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MMP2 Monoclonal antibody
MMP2 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, rat, mouse, pig
WB, IHC, IF, ELISA
Cat no : 66366-1-Ig
Validation Data Gallery
|Positive WB detected in||HepG2 cells, human placenta tissue, pig heart tissue, rat heart tissue, human heart tissue, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Sample-dependent, check data in validation data gallery|
|WB||See 40 publications below|
|IHC||See 5 publications below|
|IF||See 3 publications below|
66366-1-Ig targets MMP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
|Tested Reactivity||human, rat, mouse, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MMP2 fusion protein Ag25039|
|Full Name||matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|
|Calculated molecular weight||72 kDa|
|Observed molecular weight||55-74 kDa|
|GenBank accession number||BC002576|
|Gene ID (NCBI)||4313|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).
|Product Specific Protocols|
|WB protocol for MMP2 antibody 66366-1-Ig||Download protocol|
|IHC protocol for MMP2 antibody 66366-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.
Bioeng Transl Med
Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy.
Aging (Albany NY)
Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies.
Allergy-induced systemic inflammation impairs tendon quality.
Front Cell Dev Biol
An Advanced Systems Pharmacology Strategy Reveals AKR1B1, MMP2, PTGER3 as Key Genes in the Competing Endogenous RNA Network of Compound Kushen Injection Treating Gastric Carcinoma by Integrated Bioinformatics and Experimental Verification
Reticulocalbin3: A Ca2+ homeostasis regulator that promotes esophageal squamous cell carcinoma progression and cisplatin resistance
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Cassand (Verified Customer) (04-06-2022)
Works good for IHC
Ryan (Verified Customer) (02-28-2019)
Tissue was fixed in PFA and no antigen retrieval was used.
Ryan (Verified Customer) (01-10-2018)