|Positive WB detected in||HepG2 cells, human placenta tissue, pig heart tissue, rat heart tissue, human heart tissue, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Sample-dependent, check data in validation data gallery|
66366-1-Ig targets MMP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, rat, mouse, pig samples.
|Tested Reactivity||human, rat, mouse, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MMP2 fusion protein Ag25039|
|Full Name||matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|
|Calculated molecular weight||72 kDa|
|Observed molecular weight||55-74 kDa|
|GenBank accession number||BC002576|
|Gene ID (NCBI)||4313|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-28-2019)
Tissue was fixed in PFA and no antigen retrieval was used.
Ryan (Verified Customer) (01-10-2018)