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MMP3 Monoclonal antibody

MMP3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F5A9

Cat no : 66338-1-Ig

Synonyms

CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1



Tested Applications

Positive WB detected inPC-12 cells, human heart tissue, HeLa cells, fetal human brain tissue, COLO 320 cells, Caco-2 cells, pig heart tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66338-1-Ig targets MMP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MMP3 fusion protein Ag12359
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC074869
Gene symbol MMP3
Gene ID (NCBI) 4314
RRIDAB_2881718
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

Protocols

Product Specific Protocols
WB protocol for MMP3 antibody 66338-1-IgDownload protocol
IHC protocol for MMP3 antibody 66338-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biomaterials

Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.

Authors - Angelina D Schoenenberger
mouseIHC

J Nanobiotechnology

Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression

Authors - Yunhao Wang
humanWB,IHC

Acta Pharmacol Sin

Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.

Authors - Jia-Wei Li
humanWB

Aging (Albany NY)

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.

Authors - Jingyu Ding
mouseWB

EBioMedicine

Allergy-induced systemic inflammation impairs tendon quality.

Authors - Christine Lehner
humanWB

Front Cell Dev Biol

Reducing Inflammation and Vascular Invasion in Intervertebral Disc Degeneration via Cystathionine-γ-Lyase Inhibitory Effect on E-Selectin.

Authors - Haoran Xu