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MMP9 (N-terminal) Polyclonal antibody
MMP9 (N-terminal) Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 10375-2-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, Jurkat cells, A375 cells, rat brain tissue, Raji cells, HUVEC cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human cervical cancer tissue, mouse heart tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10375-2-AP targets MMP9 (N-terminal) in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Astragalus membranaceus, hamster, human, mouse, pig, rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP9 (N-terminal) fusion protein Ag0552|
|Full Name||matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|
|Calculated molecular weight||707 aa, 78 kDa|
|Observed molecular weight||67 kDa, 92 kDa|
|GenBank accession number||BC006093|
|Gene ID (NCBI)||4318|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).
|Product Specific Protocols|
|WB protocol for MMP9 (N-terminal) antibody 10375-2-AP||Download protocol|
|IHC protocol for MMP9 (N-terminal) antibody 10375-2-AP||Download protocol|
|IF protocol for MMP9 (N-terminal) antibody 10375-2-AP||Download protocol|
|IP protocol for MMP9 (N-terminal) antibody 10375-2-AP||Download protocol|
|FC protocol for MMP9 (N-terminal) antibody 10375-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hongxue (Verified Customer) (01-12-2021)
Clear band for WB
Kyosuke (Verified Customer) (06-12-2019)
I am working on aneurysm study and use this for IHC staining on mouse aneurysm section. It works very well.
JIMMY (Verified Customer) (04-15-2019)
This antibody detects MMP9 expressing cells in the spinal cord of C57BL/6 EAE mice. The imaging is quiet clear with this antibody.
Lucy (Verified Customer) (03-08-2019)
Reacts mainly to human MMP9 but slightly cross-reactive to human MMP2