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MMP9 (N-terminal) Polyclonal antibody

MMP9 (N-terminal) Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10375-2-AP

Synonyms

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9, MMP-9, MMP9 (N-terminal)



Tested Applications

Positive WB detected inJurkat cells, LNCaP cells, Raji cells, MDA-MB-231 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10375-2-AP targets MMP9 (N-terminal) in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, fish, hamster, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP9 (N-terminal) fusion protein Ag0552
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Calculated molecular weight 707 aa, 78 kDa
Observed molecular weight 67 kDa, 92 kDa
GenBank accession numberBC006093
Gene symbol MMP9
Gene ID (NCBI) 4318
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Protocols

Product Specific Protocols
WB protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
IHC protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
IF protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
FC protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

Authors - Haojie Jin
mouseWB,IHC

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian
humanWB

Mol Cancer

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Authors - Bing Yao
humanWB, IHC

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui

ACS Nano

Nanozyme-Based Bandage with Single-Atom Catalysis for Brain Trauma.

Authors - Ruijuan Yan
humanIHC

J Thorac Oncol

Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling.

Authors - Baoping Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (07-11-2022)

The MMP9 antibody detects nicely a protein with a size of about 100 kD and the blot looks like the blots on the product page

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse macrophage cell line J774
MMP9 (N-terminal) Antibody Western Blot validation (1:1000 dilution) in mouse macrophage cell line J774 (Cat no:10375-2-AP)
FH

Hongxue (Verified Customer) (01-12-2021)

Clear band for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
MMP9 (N-terminal) Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:10375-2-AP)
FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on aneurysm study and use this for IHC staining on mouse aneurysm section. It works very well.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: mouse aorta
FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects MMP9 expressing cells in the spinal cord of C57BL/6 EAE mice. The imaging is quiet clear with this antibody.

  • Applications: Immunofluorescence,
FH

Lucy (Verified Customer) (03-08-2019)

Reacts mainly to human MMP9 but slightly cross-reactive to human MMP2

  • Applications: Western Blot,
MMP9 (N-terminal) Antibody Western Blot, validation ( dilution) in (Cat no:10375-2-AP)