MOBP Polyclonal antibody

MOBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12472-1-AP

Synonyms

MOBP

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Validation Data Gallery

• 

  WB analysis of mouse brain using 12472-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12472-1-AP (MOBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12472-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12472-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12472-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12472-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12472-1-AP targets MOBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOBP fusion protein Ag3156
• Full Name: myelin-associated oligodendrocyte basic protein
• Calculated Molecular Weight: 183 aa, 21 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC022471
• Gene Symbol: MOBP
• Gene ID (NCBI): 4336
• RRID: AB_10644161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Neurobiol Dis; Inhibition of CXCR2 enhances CNS remyelination via modulating PDE10A/cAMP signaling pathway; Authors - Cheng Ju
mouse	WB	Glia; ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.; Authors - Lukasz Mateusz Szewczyk
human	IHC	Bioengineered; Four specific biomarkers associated with the progression of glioblastoma multiforme in older adults identified using weighted gene co-expression network analysis; Authors - Yushi Yang