MRPL33 Polyclonal antibody

MRPL33 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23870-1-AP

Synonyms

C2orf1, L33mt, MRP L33, MRPL33, RPL33L

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Validation Data Gallery

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  IHC staining of human kidney using 23870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23870-1-AP (MRPL33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 23870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23870-1-AP (MRPL33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 23870-1-AP

  Immunofluorescent analysis of HepG2 cells using 23870-1-AP (MRPL33 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23870-1-AP is MRPL33 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL33 fusion protein Ag20921
• Full Name: mitochondrial ribosomal protein L33
• Calculated Molecular Weight: 65 aa, 8 kDa
• GenBank Accession Number: BC009475
• Gene Symbol: MRPL33
• Gene ID (NCBI): 9553
• RRID: AB_2879345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL33 is a mitochondrial ribosomal protein. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.