MRPS22 Polyclonal antibody
MRPS22 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10984-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, rat spleen tissue, mouse liver tissue, mouse spleen tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 18 publications below |
Product Information
10984-1-AP targets MRPS22 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MRPS22 fusion protein Ag1421 |
Full Name | mitochondrial ribosomal protein S22 |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 38 kDa |
GenBank accession number | BC009296 |
Gene symbol | MRPS22 |
Gene ID (NCBI) | 56945 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRPS22, also named as C3orf5, RPMS22 and S22mt, is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5). MRPS22 is a 360-amino-acid protein that has been found in a limited number of species, having no ortholog in fungi or prokaryotes. The exact location of MRPS22 in the three dimensional structure of the ribosome and its exact function are currently unknown. Recently, a MRPS22 defect was shown to strongly hamper assembly of the small ribosomal subunit, whereas assembly of the large subunit and part of the small subunit were only mildly affected.(PMID:21189481)
Protocols
Product Specific Protocols | |
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WB protocol for MRPS22 antibody 10984-1-AP | Download protocol |
IHC protocol for MRPS22 antibody 10984-1-AP | Download protocol |
IP protocol for MRPS22 antibody 10984-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nucleic Acids Res Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB. | ||
Cell Rep MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. | ||
Cell Rep Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival. | ||
PLoS One Stomatin-like protein 2 deficiency results in impaired mitochondrial translation. | ||
Hum Mol Genet Mutations in the mitochondrial ribosomal protein MRPS22 lead to primary ovarian insufficiency. |