MRPS22 Polyclonal antibody

MRPS22 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10984-1-AP

Synonyms

C3orf5, COXPD5, GIBT, GK002, MRP S22, MRPS22, RPMS22, S22mt

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Validation Data Gallery

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  WB analysis of Jurkat using 10984-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10984-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10984-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 10984-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10984-1-AP (MRPS22 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 10984-1-AP

  IP Result of anti-MRPS22 (IP:10984-1-AP, 4ug; Detection:10984-1-AP 1:300) with Jurkat cells lysate 2400ug.

• 

  IHC staining of human breast cancer using 10984-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10984-1-AP (MRPS22 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, rat spleen tissue, mouse liver tissue, mouse spleen tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 18 publications below

Product Information

10984-1-AP targets MRPS22 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS22 fusion protein Ag1421
• Full Name: mitochondrial ribosomal protein S22
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 38 kDa
• GenBank accession number: BC009296
• Gene symbol: MRPS22
• Gene ID (NCBI): 56945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MRPS22, also named as C3orf5, RPMS22 and S22mt, is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5). MRPS22 is a 360-amino-acid protein that has been found in a limited number of species, having no ortholog in fungi or prokaryotes. The exact location of MRPS22 in the three dimensional structure of the ribosome and its exact function are currently unknown. Recently, a MRPS22 defect was shown to strongly hamper assembly of the small ribosomal subunit, whereas assembly of the large subunit and part of the small subunit were only mildly affected.(PMID:21189481)

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB.; Authors - Fenna Hensen
mouse	WB	Cell Rep; MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.; Authors - Jakob D Busch
mouse	WB	Cell Rep; Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.; Authors - Christopher Lowden
mouse	WB	PLoS One; Stomatin-like protein 2 deficiency results in impaired mitochondrial translation.; Authors - Panagiotis Mitsopoulos
human	WB	FEBS J; Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.; Authors - Francesco Bruni
human	WB	Hum Mol Genet; Mutations in the mitochondrial ribosomal protein MRPS22 lead to primary ovarian insufficiency.; Authors - Anlu Chen