Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16133-1-AP targets MUL1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9119 Product name: Recombinant human MUL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-239 aa of BC010101 Sequence: SVYRQKARVSQELKGAKKVHLGEDLKSILSEAPGKCVPYAVIEGAVRSVKETLNSQFVENCKGVIQRLTLQEHKMVWNRTTHLWNDCSKIIHQRTNTVPFDLVPHEDGVDVAVRVLKPLDSVDLGLETVYEKFHPSIQSFTDVIGHYISGERPKGIQETEEMLKVGATLTGVGELVLDNNSVRLQPPKQGMQYYLSSQDFDSLLQRQESSVRL Predict reactive species |
| Full Name | mitochondrial E3 ubiquitin ligase 1 |
| Calculated Molecular Weight | 352 aa, 40 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC010101 |
| Gene Symbol | MUL1 |
| Gene ID (NCBI) | 79594 |
| RRID | AB_2147111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969V5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitochondrial ubiquitin ligase 1 (MUL1),MAPL, MULAN, or gGIDE, was identified as an E3 protein ligase by three independent groups. Work in mammalian systems shows that MUL1 has small ubiquitin-like modifier (SUMO) ligase activity, stabilizing Drp1, or ubiquitin ligase activity, degrading Mfn. MUL1 expression in mammalian cells results in smaller and more fragmented mitochondria.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MUL1 antibody 16133-1-AP | Download protocol |
| WB protocol for MUL1 antibody 16133-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MUL1 antibody (Cat# 16133-1-AP) has 16 citations across 14 journals, including Nature Communications, Cell Death & Disease, Journal of Experimental & Clinical Cancer Research, International Journal of Biological Sciences, and European Journal of Pharmacology. Research fields span mitochondrial dynamics, cancer (gastric, lung, colorectal, bladder, ovarian), ischemia/reperfusion injury, diabetic neuropathy, and obesity.
| Species | Application | Title |
|---|---|---|
Bone Res The mitochondrial E3 ligase MAPL SUMOylates Drp1 to facilitate mitochondrial fission in intervertebral disc degeneration.
| ||
Nat Commun A mitochondria-driven quality control mechanism for peroxisomal membrane proteins. | ||
J Exp Clin Cancer Res PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer | ||
Cell Death Dis CRTAC1 enhances the chemosensitivity of non-small cell lung cancer to cisplatin by eliciting RyR-mediated calcium release and inhibiting Akt1 expression | ||
Cell Death Dis SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction. | ||
Int J Biol Sci SUMO E3 ligase MUL1 inhibits lymph node metastasis of bladder cancer by mediating mitochondrial HSPA9 translocation |
