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  • KD/KO Validated

MUTYH Polyclonal antibody

MUTYH Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19650-1-AP

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH



Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, Jurkat cells, K-562 cells, mouse thymus tissue, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19650-1-AP is MUTYH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUTYH fusion protein Ag7526
Full Name mutY homolog (E. coli)
Calculated molecular weight 60 kDa
Observed molecular weight 53-64 kDa
GenBank accession numberBC003178
Gene symbol MUTYH
Gene ID (NCBI) 4595
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Protocols

Product Specific Protocols
WB protocol for MUTYH antibody 19650-1-APDownload protocol
IHC protocol for MUTYH antibody 19650-1-APDownload protocol
IP protocol for MUTYH antibody 19650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease

Authors - Yuri Mizuno
humanWB

Oncol Rep

Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.

Authors - Yanxia Guo
  • KD Validated
humanWB

Cell Death Dis

Potent and specific MTH1 inhibitors targeting gastric cancer.

Authors - Wenjuan Zhou