MUTYH Polyclonal antibody

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• KD/KO Validated

MUTYH Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 19650-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

• 

  WB analysis of HeLa using 19650-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 19650-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 19650-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 19650-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 19650-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 19650-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 19650-1-AP

  IP result of anti-MUTYH (IP:19650-1-AP, 4ug; Detection:19650-1-AP 1:500) with HeLa cells lysate 3440ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, Jurkat cells, K-562 cells, mouse thymus tissue, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse liver tissue, human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19650-1-AP targets MUTYH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7526

  Product name: Recombinant human MUTYH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 187-535 aa of BC003178

  Sequence: MPRTAETLQQLLPGVGRYTAGAIASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPATHLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ

• Full Name: mutY homolog (E. coli)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 53-64 kDa
• GenBank Accession Number: BC003178
• Gene Symbol: MUTYH
• Gene ID (NCBI): 4595
• RRID: AB_10694143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Publications

What published studies show

The MUTYH antibody (Cat# 19650-1-AP) has 5 total citations across journals including Cell Death & Disease, Oxidative Medicine and Cellular Longevity, Scientific Reports, Oncology Reports, and Genes and Environment. Research fields span gastric cancer, Alzheimer's disease and neurogenesis, bladder cancer, esophageal squamous cell carcinoma, and DNA repair/mutagenesis mechanisms. All citations used the antibody for Western blot applications in human or mouse samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; Potent and specific MTH1 inhibitors targeting gastric cancer.; Authors - Wenjuan Zhou
mouse	WB	Oxid Med Cell Longev; MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease; Authors - Yuri Mizuno
human	WB	Sci Rep; Hinokiflavone is a novel CK2 inhibitor promoting apoptosis and synergizing with chemotherapeutic agents in cisplatin resistant bladder cancer cells.; Authors - Tsung-Han Hsieh
human	WB	Oncol Rep; Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.; Authors - Yanxia Guo ; KD Validated
human	WB	Genes Environ; Action-at-a-distance mutations by 8-oxo-7,8-dihydroguanine: adenine pair triggered by MUTYH.; Authors - Ruriko Fukushima ; KD Validated