Validation Data Gallery
|Positive WB detected in||HeLa cells, COLO 320 cells, Jurkat cells, K-562 cells, mouse thymus tissue, Raji cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19650-1-AP is MUTYH Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MUTYH fusion protein Ag7526|
|Full Name||mutY homolog (E. coli)|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||53-64 kDa|
|GenBank accession number||BC003178|
|Gene ID (NCBI)||4595|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.
Oxid Med Cell Longev
MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease
Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.
Cell Death Dis
Potent and specific MTH1 inhibitors targeting gastric cancer.