MUTYH Polyclonal antibody

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  WB analysis of HeLa using 19650-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19650-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19650-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19650-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 19650-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19650-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19650-1-AP

  IP result of anti-MUTYH (IP:19650-1-AP, 4ug; Detection:19650-1-AP 1:500) with HeLa cells lysate 3440ug.

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  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

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  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

MUTYH Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 19650-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, Jurkat cells, K-562 cells, mouse thymus tissue, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse liver tissue, human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

19650-1-AP targets MUTYH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7526

  Product name: Recombinant human MUTYH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 187-535 aa of BC003178

  Sequence: MPRTAETLQQLLPGVGRYTAGAIASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPATHLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ

• Full Name: mutY homolog (E. coli)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 53-64 kDa
• GenBank Accession Number: BC003178
• Gene Symbol: MUTYH
• Gene ID (NCBI): 4595
• RRID: AB_10694143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease; Authors - Yuri Mizuno
human	WB	Oncol Rep; Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.; Authors - Yanxia Guo ; KD Validated
human	WB	Cell Death Dis; Potent and specific MTH1 inhibitors targeting gastric cancer.; Authors - Wenjuan Zhou