MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A2

Cat no : 60003-2-Ig

Synonyms

EQKLISEEDL, myc, Myc tag, MYC-tag


"MYC tag Antibodies" Comparison

View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • WB analysis of recombinant proein using 60003-2-Ig

    WB analysis of recombinant proein using 60003-2-Ig

    Western blot of Myc-tagged fusion protein with anti-Myc-tag (60003-2-Ig) at various dilutions.

  • IP experiment of Transfected HEK-293 using 60003-2-Ig

    IP experiment of Transfected HEK-293 using 60003-2-Ig

    IP Result of anti-MYC tag (IP:60003-2-Ig, 4ug; Detection:60003-2-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.

  • IF Staining of Transfected HEK-293 using 60003-2-Ig

    IF Staining of Transfected HEK-293 using 60003-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (60003-2-Ig, Clone: 1A5A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).


Tested Applications

Positive WB detected inrecombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

60003-2-Ig targets MYC tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name MYC tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Protocols

Product Specific Protocols
IF protocol for MYC tag antibody 60003-2-IgDownload protocol
IP protocol for MYC tag antibody 60003-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP, WB

Am J Respir Crit Care Med

Loss of DP1 Aggravates Vascular Remodeling in Pulmonary Arterial Hypertension via mTORC1 Signaling.

Authors - Yuhu He

Hepatology

The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop.

Authors - Xue Feng

Nat Commun

UHRF1 suppresses retrotransposons and cooperates with PRMT5 and PIWI proteins in male germ cells.

Authors - Juan Dong

Nat Commun

Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-β signalling pathways.

Authors - Biao Ma

Nucleic Acids Res

The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.

Authors - Huiwu Ouyang
WB

Cell Death Differ

CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma.

Authors - Yuanqi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Isabelle Cristine (Verified Customer) (01-14-2022)

The antibody works generally well for the detection of MYC-tagged proteins.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293 cells
MYC tag Antibody Western Blot validation (1:500 dilution) in HEK293 cells (Cat no:60003-2-Ig)
FH

Tom (Verified Customer) (02-10-2021)

HEK293T cells were transfected with a Myc-tagged construct. The 60003-2-Ig antibody (1:2000) resulted in a lot of background. An alternative antibody was used alongside this one, with a stronger band and less background being achieved.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 2000
  • Cell Tissue Type: HEK293
MYC tag Antibody Western Blot validation (1 in 2000 dilution) in HEK293 (Cat no:60003-2-Ig)
FH

Alinda (Verified Customer) (10-09-2019)

This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST.This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK cells