"MYC tag Antibodies" Comparison
View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||recombinant protein|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
60003-2-Ig targets MYC tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
|Tested Reactivity||recombinant protein|
|Host / Isotype||Mouse / IgG1|
|Full Name||MYC tag|
|Gene ID (NCBI)|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Am J Respir Crit Care Med
Loss of DP1 Aggravates Vascular Remodeling in Pulmonary Arterial Hypertension via mTORC1 Signaling.
The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop.
UHRF1 suppresses retrotransposons and cooperates with PRMT5 and PIWI proteins in male germ cells.
Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-β signalling pathways.
Nucleic Acids Res
The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.
Cell Death Differ
CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Isabelle Cristine (Verified Customer) (01-14-2022)
The antibody works generally well for the detection of MYC-tagged proteins.
Tom (Verified Customer) (02-10-2021)
HEK293T cells were transfected with a Myc-tagged construct. The 60003-2-Ig antibody (1:2000) resulted in a lot of background. An alternative antibody was used alongside this one, with a stronger band and less background being achieved.
Alinda (Verified Customer) (10-09-2019)
This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST.This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better