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CoraLite® Plus 488-conjugated MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5A2

Cat no : CL488-60003

Synonyms

EQKLISEEDL

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite® Plus 488-conjugated MYC tag Monoclonal a (CL488-60003)

    IF Staining of Transfected HEK-293 using CL488-60003

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CL488-60003 (MYC tag antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite® Plus 488-conjugated MYC tag Monoclonal a (CL488-60003)

    IF Staining of Transfected HEK-293 using CL488-60003

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 488 MYC tag antibody (CL488-60003, Clone: 1A5A2 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-60003 targets MYC tag in IF/ICC applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name MYC tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883088
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYC tag antibody CL488-60003Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Oncogenesis

SLC26A3/NHERF2-IκB/NFκB/p65 feedback loop suppresses tumorigenesis and metastasis in colorectal cancer

Authors - Chunlin Lin
View Article