"MYC tag Antibodies" Comparison
View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Recombinant protein protein, Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16286-1-AP targets MYC tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, pig, monkey, recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9409 Product name: Recombinant MYC-tag protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: EQKLISEEDLEQKLISEEDLEQKLISEEDL Predict reactive species |
| Full Name | MYC tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11182162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | MYCTAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.
What is the molecular weight of myc?
1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).
What are the applications for myc tag?
The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106).
What is the structure of myc tag?
The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IP protocol for MYC tag antibody 16286-1-AP | Download protocol |
| WB protocol for MYC tag antibody 16286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYC tag Polyclonal antibody (Cat# 16286-1-AP) has accumulated 785 citations. Publications appear in high-impact journals including Nature, Science, Cell, Cancer Cell, Nature Communications, Molecular Cell, Autophagy, Cell Death & Differentiation, Advanced Science, and Science Advances. Research fields span oncology (various cancer types), autophagy, ferroptosis, innate and adaptive immunity, metabolism, neurodegeneration, cardiology, virology, DNA damage repair, epigenetics, and plant biology, reflecting its broad utility as a detection tool for MYC-tagged recombinant proteins across diverse experimental systems.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. | ||
Signal Transduct Target Ther Glucosidase alpha neutral C promotes influenza virus replication by inhibiting proteosome-dependent degradation of hemagglutinin | ||
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Signal Transduct Target Ther The E3 ligase RNF5 restricts SARS-CoV-2 replication by targeting its envelope protein for degradation | ||
Signal Transduct Target Ther BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability. |
Reviews
What customers say
The 15 reviews are overwhelmingly positive, with 13 five-star ratings. Users report strong, specific signals with low background in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation across diverse cell types and organisms. Several highlight ease of use and good value for routine applications. A minority noted some nonspecific bands or background, particularly at higher dilutions or in complex samples like microalgae lysates. Overall, reviewers recommend this antibody for detecting Myc-tagged proteins.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ricardo (Verified Customer) (09-10-2026) | The antibody showed good recognition of the recombinant protein containing the Myc tag. However, several nonspecific bands were also observed in the WT microalgae sample.
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Mounika (Verified Customer) (03-24-2026) | Very productive Antibody , saved tons of time in our project!
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Larissa (Verified Customer) (11-06-2025) | The MYC tag Rabbit Polyclonal Antibody performed well in our assays, showing strong and specific signal with low background. Easy to use and good value for routine applications.
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Prakash (Verified Customer) (10-28-2025) | working good in western blot
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RASHMI (Verified Customer) (08-26-2024) | I used this antibody recently for ABC staining, it worked great, highly recommended.
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RASHMI (Verified Customer) (07-26-2024) | used this product, highly recommended
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Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot, IF, IP
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Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot and IF and IP
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Amy (Verified Customer) (03-05-2023) | Detected overexpressed Mac-tagged proteins in HEK293T cell lysates but with a fair few background bands.
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James (Verified Customer) (11-30-2022) | Very good antibody
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Boyan (Verified Customer) (08-03-2022) | gives a good signal in IF
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Chahat (Verified Customer) (06-08-2022) | The myc antibody gives clear staining!
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WEI (Verified Customer) (03-08-2022) | Good antibody for detecting overexpression proteins in 293 cells.
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Minze (Verified Customer) (01-11-2022) | it works well for immunofluorecence so far.
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Phoebe (Verified Customer) (03-01-2019) | This antibody was very clean in a western blot to detect Myc-tagged pol2 in S cerevisiae whole cell extract and chromatin fractionation samples.
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