MYCBP Polyclonal antibody

• Featured Product
• KD/KO Validated

MYCBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12022-1-AP

Synonyms

c-Myc-binding protein, Associate of Myc 1, AMY-1, AMY1, AMY 1

---

Validation Data Gallery

• 

  WB analysis of human heart using 12022-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12022-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12022-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12022-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 12022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12022-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYCBP antibody 12022-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Tested Applications

• Positive WB detected in: human heart tissue, A549 cells, human kidney tissue, human lung tissue, human placenta tissue, HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

12022-1-AP targets MYCBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYCBP fusion protein Ag2651
• Full Name: c-myc binding protein
• Calculated Molecular Weight: 103 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC008686
• Gene Symbol: MYCBP
• Gene ID (NCBI): 26292
• RRID: AB_2148722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.

Publications

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
mouse	WB	Acta Physiol (Oxf); A dual role of miR-22 in rhabdomyolysis-induced acute kidney injury.; Authors - S Mathia
mouse	WB	J Cell Mol Med; Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization.; Authors - Yongqi Wang
human	WB	Oncol Lett; Overexpression of MYC binding protein promotes invasion and migration in gastric cancer.; Authors - Lijie Gong ; KD Validated