CoraLite®594-conjugated MYH10 Monoclonal antibody

MYH10 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2H8G7

Cat no : CL594-67243

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

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Validation Data Gallery

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  IF Staining of mouse cerebellum using CL594-67243

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.

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  IF Staining of mouse cerebellum using CL594-67243

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: mouse cerebellum tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67243 targets MYH10 in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYH10 fusion protein Ag17069
• Full Name: myosin, heavy chain 10, non-muscle
• Calculated Molecular Weight: 1985 aa, 230 kDa
• Observed Molecular Weight: 200-230 kDa
• GenBank Accession Number: BC150634
• Gene Symbol: MYH10
• Gene ID (NCBI): 4628
• RRID: AB_2920102
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.