MYL12B Polyclonal antibody

MYL12B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10324-1-AP

Synonyms

SHUJUN-1, SHUJUN 1, Myosin regulatory light chain 2-B, smooth muscle isoform, MYLC2B, MRLC2

---

Validation Data Gallery

• 

  WB analysis using 10324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10324-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10324-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10324-1-AP (MYL12B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10324-1-AP (MYL12B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissues
• Positive IHC detected in: human colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

10324-1-AP targets MYL12B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYL12B fusion protein Ag0398
• Full Name: myosin, light chain 12B, regulatory
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC004994
• Gene Symbol: MYL12B
• Gene ID (NCBI): 103910
• RRID: AB_2166830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL12B is a regulatory subunit of myosin and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. There are two groups of residues on the MYL12B that are phosphorylated by distinct kinases and have contrasting effects on myosin II biophysical properties.Phosphorylation at Thr18/Ser19 essentially "activates" the myosin molecule to produce force. The second group of phosphorylated residues is at the N-terminus of the MYL12B at Ser1, Ser2 and Thr9 and causes inhibitory effect on myosin activity.(PMID: 22136066)

Publications

Species	Application	Title
human	WB	Mol Biol Cell; Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells.; Authors - Breckenridge Mark T MT
human	WB	Biochemistry (Mosc); Western Blotting-Based Quantitative Measurement of Myosin II Regulatory Light Chain Phosphorylation in Small Amounts of Non-muscle Cells.; Authors - O A Kazakova
human	WB,IF	BMC Cell Biol; Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells.; Authors - Beach Jordan R JR
human	WB	J Pept Sci; Design of peptidase-resistant peptide inhibitors of myosin light chain kinase.; Authors - Asker Y Khapchaev
human	WB	Biochemistry (Mosc); Long-Term Experimental Hyperglycemia Does Not Impair Macrovascular Endothelial Barrier Integrity and Function in vitro; Authors - Asker Y Khapchaev