MYLK Polyclonal antibody

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• MYLK Polyclonal antibody (21642-1-AP)

MYLK Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 21642-1-AP Copied!

Publications (58)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Synonyms

EC:2.7.11.18, Kinase-related protein, KRP, MLCK, MLCK1

• 

  WB analysis of mouse uterus using 21642-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse large intestine using 21642-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse uterus using 21642-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 21642-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 21642-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse ovary using 21642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using MYLK antibody (21642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human rectal cancer using 21642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human rectal cancer tissue using MYLK antibody (21642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 21642-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK antibody (21642-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse large intestine tissue, mouse uterus tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse ovary tissue, human rectal cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21642-1-AP targets MYLK in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16306

  Product name: Recombinant human MYLK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1748-1914 aa of BC113456

  Sequence: TGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE

• Full Name: myosin light chain kinase
• Calculated Molecular Weight: 1914 aa, 211 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC113456
• Gene Symbol: MYLK
• Gene ID (NCBI): 4638
• RRID: AB_10734590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15746
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.

Publications

What published studies show

Anti-MYLK antibody (Cat# 21642-1-AP) has 58 citations published in journals including Nature Aging, Advanced Science, Journal of Hepatology, Cancer Letters, International Journal of Biological Sciences, Phytomedicine, Journal of Neuroinflammation, and Mucosal Immunology, among others. Research fields span intestinal barrier biology, gut microbiota, neuroinflammation and blood-brain barrier integrity, cancer (hepatocellular, colorectal, gastric), vascular smooth muscle pathology, pulmonary injury, smooth muscle contraction, ferroptosis, and pharmacological studies including traditional Chinese medicine.

Automatically generated from the 58 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Hepatol; eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma; Authors - Suiqing Zhou ; KD Validated
mouse	WB	Nat Aging; Senescent macrophages induce ferroptosis in skeletal muscle and accelerate osteoarthritis-related muscle atrophy; Authors - Wei Xiang
human	IHC	Adv Sci (Weinh); Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway; Authors - Jiayu Wang
rat	WB	Adv Sci (Weinh); Chlorogenic Acid Ameliorates Post-Infectious Irritable Bowel Syndrome by Regulating Extracellular Vesicles of Gut Microbes; Authors - Cihua Zheng
human	IF	Research (Wash D C); The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling; Authors - Xi Chen
mouse	WB	J Control Release; Antibacterial hyaluronic acid hydrogel with sustained release of larazotide as effective colitis treatment; Authors - Fengnan Yu