MYLK Polyclonal antibody

• 

  WB analysis of mouse uterus using 21642-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse large intestine using 21642-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 21642-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21642-1-AP (MYLK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 21642-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21642-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21642-1-AP (MYLK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse ovary using 21642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using MYLK antibody (21642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human rectal cancer using 21642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human rectal cancer tissue using MYLK antibody (21642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21642-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK antibody (21642-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MYLK Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 21642-1-AP

Publications(38)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Synonyms

MLCK108, MLCK1, MLCK, KRP, Kinase-related protein

---

Tested Applications

• Positive WB detected in: mouse uterus tissue, mouse large intestine tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse ovary tissue, human rectal cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

21642-1-AP targets MYLK in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16306

  Product name: Recombinant human MYLK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1748-1914 aa of BC113456

  Sequence: TGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE

• Full Name: myosin light chain kinase
• Calculated Molecular Weight: 1914 aa, 211 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC113456
• Gene Symbol: MYLK
• Gene ID (NCBI): 4638
• RRID: AB_10734590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15746
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Adv Sci (Weinh); Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway; Authors - Jiayu Wang
human	IF	Research (Wash D C); The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling; Authors - Xi Chen
human	WB	Mucosal Immunol; Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment.; Authors - R Sumagin
pig	WB	Int J Biol Macromol; Proline rich-39 (PR-39) antimicrobial protein alleviated lipopolysaccharide-induced intestinal barrier dysfunction in piglets by altering intestinal flora associated bile acid metabolism and in turn regulating TGR-5/NF-κB/MLCK/MLC pathway; Authors - Zhineng Liu
mouse	WB,IHC	Int J Biol Sci; Aryl Hydrocarbon Receptor Activation Modulates Intestinal Epithelial Barrier Function by Maintaining Tight Junction Integrity.; Authors - Min Yu
rat	IF	FEBS J; Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction.; Authors - Wenxuan Xu