Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, Jurkat cells, A549 cells, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21173-1-AP targets MYLK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15424 Product name: Recombinant human MYLK2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-300 aa of BC069627 Sequence: MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVC Predict reactive species |
| Full Name | myosin light chain kinase 2 |
| Calculated Molecular Weight | 596 aa, 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC069627 |
| Gene Symbol | MYLK2 |
| Gene ID (NCBI) | 85366 |
| RRID | AB_10733123 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H1R3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYLK2, also named as MLCK2, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. MYLK2 catalyze the reaction: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. MYLK2 is implicated in the level of global muscle contraction and cardiac function. MYLK2 phosphorylates a specific serine in the N-terminus of a myosin light chain. Mutation of MYLK2 will cause the cardiomyopathy familial hypertrophic (CMH).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYLK2 antibody 21173-1-AP | Download protocol |
| WB protocol for MYLK2 antibody 21173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYLK2 Polyclonal antibody (Cat# 21173-1-AP) has 4 citations across four journals: iScience, Microbiology Spectrum, Frontiers in Genetics, and Experimental and Therapeutic Medicine. The associated research fields include hepatic stellate cell chemotaxis in liver injury, inflammation and smooth muscle regulation in ulcerative colitis, prostate adenocarcinoma (PRAD) treatment, and pancreatic barrier dysfunction in hypertriglyceridemia-induced pancreatitis. All citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
iScience SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury. | ||
Microbiol Spectr Berberine Depresses Inflammation and Adjusts Smooth Muscle to Ameliorate Ulcerative Colitis of Cats by Regulating Gut Microbiota | ||
Front Genet Impact of ITH on PRAD patients and feasibility analysis of the positive correlation gene MYLK2 applied to PRAD treatment | ||
Exp Ther Med Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia-induced pancreatitis in vitro. |
