Myocilin Monoclonal antibody

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  WB analysis of pig heart using 60357-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 60357-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 60357-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 60357-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens).

Myocilin Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60357-1-Ig

Clone No.4B1F6

Publications(10)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF, ELISA

Synonyms

GLC1A, GPOA, JOAG, JOAG1, MYOC, myocilin, TIGR

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Tested Applications

• Positive WB detected in: pig heart tissue, human skeletal muscle tissue, human spleen tissue, rat heart tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

60357-1-Ig targets Myocilin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5492

  Product name: Recombinant human MYOC protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 154-504 aa of BC029261

  Sequence: EEKKRLRQENENLARRLESSSQEVARLRRGQCPQTRDTARAVPPGSREVSTWNLDTLAFQELKSELTEVPASRILKESPSGYLRSGEGDTGCGELVWVGEPLTLRTAETITGKYGVWMRDPKPTYPYTQETTWRIDTVGTDVRQVFEYDLISQFMQGYPSKVHILPRPLESTGAVVYSGSLYFQGAESRTVIRYELNTETVKAEKEIPGAGYHGQFPYSWGGYTDIDLAVDEAGLWVIYSTDEAKGAIVLSKLNPENLELEQTWETNIRKQSVANAFIICGTLYTVSSYTSADATVNFAYDTGTGISKTLTIPFKNRYKYSSMIDYNPLEKKLFAWDNLNMVTYDIKLSKM

• Full Name: myocilin, trabecular meshwork inducible glucocorticoid response
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC029261
• Gene Symbol: Myocilin
• Gene ID (NCBI): 4653
• RRID: AB_2881465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99972
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IHC	Stem Cell Res Ther; Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.; Authors - Yourka D Tchoukalova
rat	WB	Exp Eye Res; Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork; Authors - Dingwen Xu
mouse	WB,IF	bioRxiv; Ocular hypertension impairs axonal transport in the optic nerve head leading to neurodegeneration in a novel Cre-inducible mouse model of myocilin glaucoma; Authors - Balasankara Reddy Kaipa
mouse	WB,IF	JCI Insight; Impaired axonal transport contributes to neurodegeneration in a Cre-inducible mouse model of myocilin-associated glaucoma; Authors - Balasankara Reddy Kaipa
human	WB	Biochem Biophys Res Commun; Identification and application of p75 neurotrophin receptor-expressing human trabecular meshwork progenitor cells.; Authors - Susumu Hara
human	WB,IF	Int Ophthalmol; The effect of ET1-CTGF mediated pathway on the accumulation of extracellular matrix in the trabecular meshwork and its contribution to the increase in IOP; Authors - Junming Wang