Filter: AllWBIHC

WB analysis of pig heart using 60357-1-Ig (same clone as 60357-1-PBS)
pig heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.
WB analysis of rat heart using 60357-1-Ig (same clone as 60357-1-PBS)
rat heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.
WB analysis of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.
WB analysis of human spleen using 60357-1-Ig (same clone as 60357-1-PBS)
human spleen tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 60357-1-Ig (same clone as 60357-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 60357-1-Ig (same clone as 60357-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 60357-1-Ig (same clone as 60357-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60357-1-PBS in a different storage buffer formulation.

Myocilin Monoclonal antibody, PBS Only

Myocilin Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60357-1-PBS

Clone No.4B1F6

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

GLC1A, GPOA, JOAG, JOAG1, MYOC, myocilin, TIGR

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60357-1-PBS targets Myocilin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity	human, mouse, pig, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5492 Product name: Recombinant human MYOC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 154-504 aa of BC029261 Sequence: EEKKRLRQENENLARRLESSSQEVARLRRGQCPQTRDTARAVPPGSREVSTWNLDTLAFQELKSELTEVPASRILKESPSGYLRSGEGDTGCGELVWVGEPLTLRTAETITGKYGVWMRDPKPTYPYTQETTWRIDTVGTDVRQVFEYDLISQFMQGYPSKVHILPRPLESTGAVVYSGSLYFQGAESRTVIRYELNTETVKAEKEIPGAGYHGQFPYSWGGYTDIDLAVDEAGLWVIYSTDEAKGAIVLSKLNPENLELEQTWETNIRKQSVANAFIICGTLYTVSSYTSADATVNFAYDTGTGISKTLTIPFKNRYKYSSMIDYNPLEKKLFAWDNLNMVTYDIKLSKM Predict reactive species
Full Name	myocilin, trabecular meshwork inducible glucocorticoid response
Calculated Molecular Weight	57 kDa
Observed Molecular Weight	50-57 kDa
GenBank Accession Number	BC029261
Gene Symbol	Myocilin
Gene ID (NCBI)	4653
RRID	AB_2881465
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q99972
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).