MYOM1-Specific Polyclonal antibody, PBS Only

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  WB analysis using 20360-1-AP (same clone as 20360-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 20360-1-AP (same clone as 20360-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 20360-1-AP (same clone as 20360-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 20360-1-AP (same clone as 20360-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYOM1-Specific antibody (20360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

MYOM1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 20360-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

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Product Information

20360-1-PBS targets MYOM1-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myomesin 1, 185kDa
• Calculated Molecular Weight: 188 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: NM_003803
• Gene Symbol: MYOM1
• Gene ID (NCBI): 8736
• RRID: AB_10696312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52179
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent. The antibody is specific to MYOM1.