Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue, mouse heart tissue |
| Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20360-1-AP targets MYOM1-Specific in WB, IHC, IF/ICC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myomesin 1, 185kDa |
| Calculated Molecular Weight | 188 kDa |
| Observed Molecular Weight | 190 kDa |
| GenBank Accession Number | NM_003803 |
| Gene Symbol | MYOM1 |
| Gene ID (NCBI) | 8736 |
| RRID | AB_10696312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52179 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent. The antibody is specific to MYOM1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYOM1-Specific antibody 20360-1-AP | Download protocol |
| IHC protocol for MYOM1-Specific antibody 20360-1-AP | Download protocol |
| WB protocol for MYOM1-Specific antibody 20360-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYOM1-Specific Polyclonal antibody (Cat# 20360-1-AP) has 8 citations published in journals including J Cachexia Sarcopenia Muscle, Int J Mol Sci, Sci Rep, Cell Signal, J Cell Mol Med, iScience, Front Physiol, and EMBO Rep. Research fields span skeletal muscle atrophy and homeostasis, cardiac hypertrophy, pulmonary hypertension, type 2 diabetes mellitus, myocardial alternative splicing, and maternal obesity-induced cardiac defects.
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle Fast Myosin Binding Protein-C Is a Vital Regulator in Young and Aged Fast Skeletal Muscle Homeostasis. | ||
Sci Rep Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. | ||
Cell Signal Long non-coding RNA Pvt1 modulates the pathological cardiac hypertrophy via miR-196b-mediated OSMR regulation. | ||
J Cell Mol Med Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration. | ||
iScience Splicing factor Srsf5 deletion disrupts alternative splicing and causes noncompaction of ventricular myocardium |
