Matrilin 2 Polyclonal antibody, PBS Only

• 

  WB analysis of NIH/3T3 using 24064-1-AP (same clone as 24064-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 24064-1-AP (same clone as 24064-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 24064-1-AP (same clone as 24064-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 24064-1-AP (same clone as 24064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24064-1-AP (Matrilin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 24064-1-AP (same clone as 24064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24064-1-AP (Matrilin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 24064-1-AP (same clone as 24064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24064-1-AP (Matrilin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

Matrilin 2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 24064-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

MATN2, Matrilin-2, UNQ193/PRO219

---

Product Information

24064-1-PBS targets Matrilin 2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21113

  Product name: Recombinant human MATN2 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 26-351 aa of BC010444

  Sequence: RSRGRSISRGRHARTHPQTALLESSCENKRADLVFIIDSSRSVNTHDYAKVKEFIVDILQFLDIGPDVTRVGLLQYGSTVKNEFSLKTFKRKSEVERAVKRMRHLSTGTMTGLAIQYALNIAFSEAEGARPLRENVPRVIMIVTDGRPQDSVAEVAAKARDTGILIFAIGVGQVDFNTLKSIGSEPHEDHVFLVANFSQIETLTSVFQKKLCTAHMCSTLEHNCAHFCINIPGSYVCRCKQGYILNSDQTTCRIQDLCAMEDHNCEQLCVNVPGSFVCQCYSGYALAEDGKRCVAVDYCASENHGCEHECVNADGSYLCQCHEGFA

• Full Name: matrilin 2
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 120-150 kDa
• GenBank Accession Number: BC010444
• Gene Symbol: Matrilin 2
• Gene ID (NCBI): 4147
• RRID: AB_2879422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: O00339
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The matrilins represent a new family of oligomeric non-collagenous extracellular matrix proteins that are assumed to act as adapter molecules connecting other proteins and proteoglycans in the extracellular matrix (PMID: 17513098). Four matrilins (matrilin-1, -2, -3, and -4) have been identified. They all share a structure made up of von Willebrand factor A domains, EGF-like domains and a coiled-coil alpha-helical module (PMID: 18386166). Matrilin-1 and matrilin-3 are expressed mainly in cartilage, while matrilin-2 and matrilin-4 show broader tissue distribution. Matrilin-2 is the largest member of the family. It incorporates into loose and dense connective tissue and becomes associated with some basement membranes (PMID: 17513098).