ChromoTek Mdm4/ HdmX-Trap Agarose

Validation Data Gallery

• 

  Pull-down of Mdm4/HdmX: Western blot I: Input, FT: Flow-through, B: Bound.

Mdm4/HdmX-Trap Agarose is an affinity resin for IP of Mdm4/HdmX-fusion proteins.

Cat No. hta

Specificity

Mdm4/HdmX

Reactivity

Human, Mouse, Hamster

Applications

IP, CoIP

Type

Nanobody

Conjugate

Agarose

Synonyms

Mdm4/ HdmX-Trap, Mdm4/HdmX-Trap, MDMX, p53-binding protein Mdm4

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Product Information

Mdm4/HdmX-Trap Agarose is an affinity resin for IP of Mdm4/HdmX-fusion proteins.

• Description: Immunoprecipitation of Mdm4/HdmX and interacting factors with Mdm4/HdmX-Trap Agarose.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP
• Specificity/Target: Double minute 4 protein (Mdm4/HdmX), human; Note that binding of Mdm4/HdmX-Trap interferes with binding of p53 to Mdm4 HdmX.
• Tested Reactivity: Human, Mouse, Hamster
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Compatibility with mass spectrometry: The Mdm4/HdmX-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631374
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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