Midkine Polyclonal antibody

Midkine Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28546-1-AP

Synonyms

ARAP, FLJ27379, MDK, Midkine, MK, MK1, NEGF2



Tested Applications

Positive WB detected inmouse embryo tissue
Positive IHC detected inhuman pancreas cancer tissue, human stomach cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28546-1-AP targets Midkine in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Midkine fusion protein Ag29185
Full Name midkine (neurite growth-promoting factor 2)
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC011704
Gene Symbol MDK
Gene ID (NCBI) 4192
RRIDAB_2881168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Midkine antibody 28546-1-APDownload protocol
IHC protocol for Midkine antibody 28546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols